Concentration of receptors for estradiol and progesterone in canine endometrium during estrus and diestrus.

Receptors for estrogen and progesterone were measured in cytosols prepared from specimens of canine endometrium obtained at late proestrus, day 4 of estrus, day 2 of diestrus, and at 10 day intervals from days 10 through 80 of diestrus. Twenty nine adult bitches were used, with 2 to 4 dogs used at each time point. Concentrations of estradiol receptors measured in endometrial cytosols from late proestrus through day 10 of diestrus were similar (mean +/- SEM: 9.

Effects of progesterone on the oviductal epithelium in estrogen-primed prepubertal beagles: light and electron microscopic observations.

A total of 45 prepubertal beagles 6 to 8 weeks of age were used to study the cytological changes that accompany regression of the oviductal epithelium. The oviductal epithelium in untreated pups consisted of undifferentiated low cuboidal cells that measured 10.3 +/- 2.

Hormonal regulation of cytoplasmic estrogen and progesterone receptors in the beagle uterus and oviduct.

The hormonal regulation of uterine and oviductal cytoplasmic estrogen and progesterone receptors was studied in immature beagles that were untreated, treated with estradiol-17 beta, or treated sequentially with estradiol and progesterone. Estradiol treatment increased the concentration of estrogen receptors in both tissues. Progesterone receptors were not detectable in the reproductive tract of untreated animals, but increased dramatically under the influence of estradiol.

Analysis of progesterone receptor binding in the ovine uterus.

The appropriate conditions for the measurement of ovine uterine cytoplasmic progesterone receptors (PR) have been determined to be 20 nM 3H-progesterone (3H-P4) with and without a 100-fold excess of non-radioactive progesterone (P4) 0-4 degrees C and 4 h of incubation. Under these conditions PR readily exchanged bound progesterone for progesterone added during the assay. This exchange occurred even when saturating concentrations of P4 were present.