[Diagnostic value of the determination of free oxyproline in hereditary and acquired collagenoses].

Oxyproline is one of the major collagen amino acids, which enables it to be regarded as a marker that reflects the catabolism of this protein. The purpose of this investigation was to ascertain the diagnostic value of free oxyproline in patients with hereditary and acquired collagenoses. The examinees were divided into 2 groups: (1) patients with hereditary collagenoses and (2) those with acquired ones.

[Diagnostic value of the determination of hyperphenylalaninemia in the newborn].

Blood enzyme immunoassay was carried out to determine the level of phenylalanine in 90 newborn infants. Elevated blood phenylalanine was found in 42 (46.7%) children.

[Methemoglobin level and its comparison with levels of globins and membrane proteins in erythrocytes of patients with beta-thalassemia].

On the basis of conducted biological-and-chemical research, the clinical-and-genetic forms of beta-thalassemia were revealed, as well as fractions and the quantity of globin chains and membrane proteins were defined. The methods of electrophoresis and of isoelectrofocusing were made use of within the case study to separate between the globin chains and the membrane proteins. Samples of venous blood obtained from one hundred and one children with beta-thalassemia and from 45 donors were tested.

[Evaluation of the use of isoelectrofocusing method for measuring glycated hemoglobin].

Measurement of HbA1c is important for the diagnosis of early forms of diabetes mellitus, hemolytic and sickle-cell anemia, as it can be used for optimal and permanent monitoring of hyperglycemia and serve as an indicator of hemolysis process. Isoelectrofocussing method (IEF) was modified for measuring HbA1c in polyacrylamide gel (pH 3.5-9.

[Familial case of methemoglobinemia associated with glucose-4-phosphate dehydrogenase deficiency].

More than 1500 umbilical blood samples from newborns were examined by modified analytical isoelectric focussing method on polyacrylamide-ampholine gels at Ph3.5-9.5 and 5.