Potential Use of Common Administration of Emulsion for Parenteral Nutrition and Vinpocetine: Compatibility Study and Prospect.

Vinpocetine (VP) is distributed after oral and intravenous administration, and its uptake in the thalamus, basal ganglia, and visual cortex. Due to poor bioavailability (~7%) and marked first-pass effect (~75%), including a short half-life (2-3 h), oral administration of VP is limited. It requires frequent administration of the drug to obtain a therapeutic effect.

Antiemetic Drugs Compatibility Evaluation with Paediatric Parenteral Nutrition Admixtures.

Chemotherapy-induced nausea and vomiting are defined as the most common of side effects of treatment and, at the same time, are very difficult to accept for patients', frequently causing changes in the therapy regimen, significantly reducing its effectiveness. Thus, an antiemetic prophylactic is essential to the provision of such a therapy for the patient. Pharmacotherapy often includes various drugs, including antiemetics, with the administration of such drugs by injection through two separate catheters being the preferred method.

Study of The Impact of Users' Features on Dimensional Allowances Resulting from the Use of Personal Protective Equipment.

Up-to-date anthropometric data on the human population are needed for designing safe and ergonomically efficient workplaces. An important determinant of safety and ergonomic comfort at work is knowledge of the value of dimensional allowances (DAs) when using personal protective equipment (PPE) as the dimensions and space occupied by workers increase. This is particularly important in environments characterized by spatial constraints.

3D Hand Scanning Methodology for Determining Protective Glove Dimensional Allowances.

There are two types of dimensional allowance (inner and external) related to two distinct areas of occupational health and safety: those being a measure of fit of personal protective equipment (PPE) and those determining the safe and comfortable human interaction with tools and machines, e.g., the latter ones result from wearing PPE increasing the dimensions of the human body and generating limitations in the work environment.

Procedure for Determining Dimensional Allowances for PPE Using 3D Scanning Methods.

The article describes the importance of dimensional allowances, which are a consequence of the use of personal protective equipment (PPE) for work safety. The method of 3D scanning was proposed for determining the dimensional allowances which has been preliminary validated. Two geometric solids (a cylinder and a cuboid) were used to approximate the minimum space around the person using PPE.

The Influence of Intraocular Lenses (IOLs) on the Properties of Filters Protecting Human Eyes against Optical Radiation in the Work Environment.

Under the specific illumination conditions of many workplaces, e.g., in the metallurgical industry, decreased lighting may impair workers' vision and, as a result, their productivity.

The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.

Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs). In our study we used in vitro generated AGEs to model glycation in vivo. We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.

The activity of protein tyrosine kinases of rat heart after ischemia and reperfusion.

Background: Protein phosphorylation plays a very important role in the modulation of signal transduction in many tissues including heart. The activities of protein tyrosine kinases (PTKs) of the heart are rather low but PTKs in cardiac myocytes could be involved in many processes including necrosis, apoptosis, and inflammation. All of them lead to heart failure and are the result of such conditions as ischemia and reperfusion.

Juvenile hormone binding protein and transferrin from Galleria mellonella share a similar structural motif.

It has been previously suggested that juvenile hormone binding protein(s) (JHBP) belongs to a new class of proteins. In the search for other protein(s) that may contain structural motifs similar to those found in JHBP, hemolymph from Galleria mellonella (Lepidoptera) was chromatographed over a Sephadex G-200 column and resulting fractions were subjected to SDS-PAGE, transferred onto nitrocellulose membrane and scanned with a monoclonal antibody, mAb 104, against hemolymph JHBP. Two proteins yielded a positive reaction with mAb 104, one corresponding to JHBP and the second corresponding to a transferrin, as judged from N-terminal amino acid sequencing staining.

Effect of high-dose growth hormone and glutamine on body composition, urine creatinine excretion, fatty acid absorption, and essential fatty acids status in short bowel patients: a randomized, double-blind, crossover, placebo-controlled study.

Background: Positive effects of high dose growth hormone and glutamine (GH + GLN) on body composition in short bowel patients have been described. Lack of effects on intestinal absorption found in some studies has been ascribed to concomitant essential fatty acid (EFA) deficiency. This study describes changes in body weight (BW) and composition, 24-h urine creatinine excretion, intestinal fatty acid absorption (total, saturated, unsaturated and EFA), and EFA status in relation to treatment with GH + GLN in 8 short bowel patients.

[Developmental osteopenia: decrease of bone mineral density or systemic impairment?].

In the quantitative evaluation of bone osteopenia is defined as a decrease of mineral density by more than 1 SD from the established normal values (age, sex, peak bone mass...

Effect of high dose growth hormone with glutamine and no change in diet on intestinal absorption in short bowel patients: a randomised, double blind, crossover, placebo controlled study.

Background: High dose growth hormone, glutamine, and a high carbohydrate diet may improve intestinal function in short bowel patients.

Aims: To investigate if growth hormone with glutamine and no change in diet improved intestinal function.

Patients And Methods: Eight short bowel patients were randomised in a double blind crossover study between placebo and growth hormone (mean 0.

Differential effects of somatostatin and its analog on protein tyrosine kinases activity in the rat pituitary and the murine colonic tumors.

The effects of the native somatostatin-14 (SST-14) and of its analog octreotide (OCT) on the activity of protein tyrosine kinases (PTK) in the normal rat anterior pituitary gland, diethylstilbestrol (DES)-induced rat pituitary tumor and murine colonic cancer Colon 38 were studied in vitro. PTK activity was estimated in tissue homogenates using gamma-[32P]ATP and poly (Glu80, Tyr20) as a substrate. It was found that both SST-14 and OCT suppressed the PTK activity in all examined tissues.

Immunoaffinity purification of juvenile hormone-binding protein from Galleria mellonella hemolymph.

Previously described methods of purification of hemolymph juvenile hormone-binding protein (hJHBP) from Lepidoptera were tedious and required multiple steps. These methods resulted in low protein yield (Kramer et al., 1976; Goodman et al.

Treatment of severe obesity with a highly selective serotonin re-uptake inhibitor as a supplement to a low calorie diet.

Inhibitors of serotonin (5HT) re-uptake have generally been successful in inducing modest but statistically significant weight reductions in clinical trials. Citalopram is a new, highly selective inhibitor of 5HT re-uptake. It is effective and safe in relieving major depression at doses up to 60 mg daily.

Substance P and its fragments affect Ca2+/calmodulin-dependent synaptosomal membrane protein phosphorylation from rat cerebral cortex.

1. We have used synaptosomal membranes to study the influence of substance P and its fragments and analogues of its C-terminal fragment on Ca2+/calmodulin-dependent synapsin I endogenous phosphorylation. 2.

The influence of substance P and its fragments on endogenous phosphorylation of synaptosomal membrane protein (synapsin) from cerebral cortex of rat brain.

1. The effects of substance P and its fragments and analogue of a C-terminal fragment on cyclic AMP-dependent phosphorylation of synapsin I in synaptosomal membranes (SM) from cerebral cortex were investigated. 2.

Characterization of anti-prostatic acid phosphatase monoclonal antibody and its medical significance.

Monoclonal antibody to purified prostatic acid phosphatase from seminal plasma was produced by fusion of spleen cells from immunized mice with the Sp2/O-Ag 14 cell line. This hybridoma-derived antibody, designated MAb-14, was classified as IgG1 immunoglobulin. The apparent affinity constant of phosphatase.

Immunoreactivity of proteolytic degradation products of human prostatic acid phosphatase.

Prostatic acid phosphatase (EC 3.1.3.

Effects in vitro of L-glutamate and kainic acid on the ATPase activities of synaptosomal membranes from different areas of rat brain.

Changes in the activities of enzymes responsible for the active transport of Na+, K+, Ca2+, Mg2+ in synaptosomal membrane (SM) preparations from the cerebral cortex, hippocampus and thalamus with hypothalamus after incubation with L-glutamate (Glu) or kainic acid (KA) were investigated. Glu stimulated Ca,Mg- and Na,K-ATPase activities in cortex but reduced the activities of all the investigated ATPases, except Na,K-ATPase in the hippocampus and thalamus with hypothalamus. KA reduced distinctly the activity of ATPases in the cortex and only slightly in the thalamus with hypothalamus, but stimulated the enzyme activities in the hippocampus.

Detection of prostatic tissue and seminal plasma peptides reacting with human seminal fluid acid phosphatase antibodies.

IgG1 monoclonal antibody to purified seminal fluid phosphatase was raised by fusion of spleen cells from immunized mice with cell line Sp2/O-Ag 14 using simple method of screening for antiphosphatase antibody secreting clones. All molecular forms of catalytically active seminal fluid phosphatase and prostatic tissue phosphatase, resolved by chromatofocusing in pH gradient, react with this monoclonal antibody and with rabbit antiserum to purified seminal fluid phosphatase. Peptides of Mr 25,000 to 76,000 and of Mr 13,000 to 76,000 were adsorbed from the prostatic tissue extract and from seminal plasma on the monoclonal antibody-Sepharose column.

Quantitative analysis of tissue distribution of Ly10-like murine alloantigen(s) with antisera and monoclonal antibodies.

Tissue distribution of non-Lyt1.1 ("Ly10-like") antigen or antigens encoded by short chromosomal segment differentiating B6-Ly-1a congenic strain from B6 strain of mice was studied by quantitative absorption of (BALB/c X B6)F1 anti B6-Ly-1a antiserum and by direct cytotoxicity of Ly-10-132-12-26 monoclonal antibody on lymphoid cell populations. Identical strain but not tissue distribution pattern does not allow to conclude whether antiserum and monoclonal antibody detect the same or closely linked antigens.

Cell surface antigens of L-1210 leukemia recognized by monoclonal antibodies.

Conventional antisera to L-1210 leukemia were being prepared in our laboratory for nearly a decade and consistently the only specificity detectable was anti Mammary Leukemia antigen (ML). Serological analysis of five monoclonal antibodies obtained following the same immunization schedule showed more diverse pattern of reactivity. Two antigens detected belong to oncofetal category.

Monoclonal anti-TL antibody recognizing a TL.2-like antigen.

We obtained a monoclonal anti-TL antibody-producing hybridoma by fusion of Sp2/0 hybrid cells with spleen cells from (B6 X A-Tlab) F1 mice immunized with ASL1 leukemia cells. The antibody, TL-22-27-17, reacts in complement-dependent cytotoxicity assay with ASL1 cells as well as with normal thymocytes of TL+ mouse strains (B6-Tlaa, A, BALB/c, DBA/2 and 129) but not with thymocytes from TL- mice (B6--Ly-1a). In addition, it did not react with lymph node cells or Concanavalin A-induced spleen blasts from B6-Tlaa mice; this result shows that TL-22-27-17 recognizes TL and not Qa-1 antigen.

Cell surface antigens of human renal cancer defined by mouse monoclonal antibodies: identification of tissue-specific kidney glycoproteins.

Seventeen monoclonal antibodies derived from fusions with spleen cells of mice immunized with established culture lines of renal cancers identified nine cell-surface antigenic systems. Six of the systems (gp160, S25, gp120r, gp120nr, gp115, and V1) represent antigens not previously described. The other three systems are related to HLA-A, -B, and -C heavy chain and A and B blood group antigens.