Prognostic and predictive evaluation of DNA content, S-phase fraction and immunophenotyping in non-Hodgkin's lymphomas in adults. A prospective study.

In non-Hodgkin's lymphoma (NHL) patients, prognostic significance of S-phase fraction (SPF) is well known, however the significance of DNA ploidy status and antigen expressions is still unclear. The purpose of the present study is to evaluate the prognostic and predictive impact of SPF, immunophenotype and DNA ploidy in prospectively analysed NHL patients. The study was performed on lymph node biopsies of 117 nodal NHL patients.

Prognostic evaluation of cutaneous malignant melanoma: a clinicopathologic and immunohistochemical study.

Background And Objectives: Depth of invasion and stage of the disease are established prognostic indicators in cutaneous malignant melanoma. The role of other parameters is still an open problem.

Methods: In 93 consecutive patients with cutaneous malignant melanoma, the level of invasion, tumor thickness, ulceration, vascular invasion, lymphoplasmocytic infiltrates, and mitotic index were evaluated by histology.

Assessment of proliferative activity, DNA values and some clinicopathologic parameters in mesenchymal tumors. Immunohistochemical and flow cytometric study.

Clinicopathologic parameters of 70 consecutive mesenchymal tumors from 63 patients were evaluated. In all these cases, the DNA content was analyzed by flow cytometry, and the expression of proliferative antigen MIB1 and p53 protein was assessed by immunohistochemistry. Our study verified the prognostic usefulness of proliferative indicators, above all MIB1-index, which strongly correlated with tumor grade and independently influenced overall survival.

Rhabdomyosarcoma. Morphologic, immunohistochemical, and DNA study.

In this study 80 cases of predominantly adult rhabdomyosarcoma are presented as follows: 20 cases of the embryonal type, 32 cases of the alveolar and 8 cases of the pleomorphic type. Additional histologic classification was performed in each type. In myotube stage the histologic picture of fetal muscles cannot be compared to the alveolar type of rhabdomyosarcoma.

Flow cytometric DNA-analysis of cells obtained with fine needle aspiration biopsy of the breast.

In 221 consecutive, unselected palpable breast lesions, evaluated by aspiration biopsy, flow cytometric DNA analysis was performed on cell material remaining in needle and syringe after smears had been prepared. DNA histograms were obtained in 186 cases, whereas in 44 samples the cell number was too low for detailed estimation of cell kinetics. Aneuploidy was observed in 71 of 90 samples where cancer cells were detected by cytology, in four of 20 samples where breast cancer could only be suspected and in 12 of those 76 where no cancer cells occurred in cytologic specimens.

Relationship of histology, DNA-values and proliferative activity in unselected breast cancer patients (a preliminary study).

Based upon a group of 108 consecutive mammary carcinomas a comparative analysis of morphological parameters, DNA-ploidy and indicators of proliferation activity was made. A correlation between Bloom-Richardson scale, mitotic index, PCNA-labeling index and DNA-index was shown. The ploidy of mammary carcinomas was significantly related to the values of proliferative fraction, as well as to PCNA-labeling index.

Flow cytometric evaluation of Ki67 proliferative antigen in primary breast cancer.

In the group of forty five primary mammary carcinomas Ki67 reactivity was evaluated with flow cytometry and immunohistochemistry. The expression of proliferative antigen, evaluated with both methods did not correlate with other indicators of proliferative activity. This result is probably associated with the physicochemical properties of breast cancer cells.

Comparative flow cytometric DNA analysis on fresh and paraffin-embedded tumor tissue in different neoplasms.

The results of flow cytometric DNA-analysis on fresh and paraffin-embedded tissue were compared in four various groups of tumors. The best correlation of the results was observed in the group of non Hodgkin's lymphomas. Comparative analysis of the ploidy on fresh and paraffin embedded tissue demonstrated discordance in particular in mammary carcinoma and malignant melanoma groups.

Flow cytometric analysis of immunophenotypic and DNA values in non-Hodgkin's B-cell lymphomas classified according to the Kiel Classification and Working Formulation.

B-cell non-Hodgkin's lymphomas classified previously according to the Kiel Classification were reclassified according to the Working Formulation. The comparative analysis of flow cytometric data was done for these two classification systems. It was established that flow cytometric data describe defined groups of malignancy more precisely in the Working Formulation, especially in case of the low grade lymphomas.

Flow cytometric analysis of high grade B-cell non-Hodgkin's lymphoma: correlation between histology, immunophenotyping and cell cycle analysis.

The immunophenotype and the DNA parameters of 21 high grade B-cell non-Hodgkin's lymphomas considered in terms of the Kiel Classification were studied by flow cytometry. The centroblastic and immunoblastic lymphomas differed in the expression of lymphocyte markers, however no characteristic immunophenotype for each lymphoma type could be distinguished. Assessment of the FSC/SSC dot plots with histology enables to distinguish subpopulations of the lymphoma cells.

Flow cytometric analysis of low grade B-cell non-Hodgkin's lymphoma: correlation between histology, immunophenotyping and cell cycle analysis.

In the present study the immunophenotyping and the DNA parameters in the group of 38 patients with B-cell non-Hodgkin's lymphoma of low grade malignancy were evaluated in relation to the Kiel Classification. The study was performed with flow cytometry. The evaluation of the FSC/SSC light scatters enables selective immunophenotyping and also corresponds to tumor histology.

Synovial sarcoma: a new approach to the histological classification and a comparison with malignant mesothelioma. Immunohistochemical study with anti-mesothelial cell serum.

Routine paraffin-embedded sections from synovial sarcomas in 70 patients were stained immunohistochemically with monoclonal anti-cytokeratin antibody and serum against mesothelial cells. Positive reactions occurred in the epithelioid cells of biphasic tumors only. On the ground of the reaction with anti-mesothelial serum a hypothetical scheme of cell differentiation in synovial sarcoma was set up.

Haemangioendothelioma epithelioides. Case report of a rare soft tissue tumour.

A case of a rare angiogenic tumor in a 77-year-old man is reported. The clinical data and histological features are analyzed. The diagnosis was confirmed by immunohistochemical studies.

Alveolar soft part sarcoma: an analysis of 8 cases. Review of the literature.

Eight cases of alveolar soft part carcinoma (ASPS) are presented. The average age of the patients was 28 years. There were 6 women and 2 men.

Pleural mesothelioma. Morphological characteristics of 17 cases.

Pleural mesotheliomas are rare tumors. Thus, only few reports are based on a material sufficient for reviewing the whole spectrum of pathomorphological features. This has stimulated this review of morphological characteristics of 17 pleural mesotheliomas.

[Morphological and clinical evaluation of non-epithelial and mixed neoplasms of the female reproductive organs. II. Leiomyosarcoma].

Among 12886 malignant neoplasms of female sex organ 34 leiomyosarcoma were found. Average age of suffering women was 50 years. Metrorrhagia and lower abdominal pain were most common symptoms reported.

Mesenchymoma malignum of the buttock.

A case of a soft-tissue tumor of the left gluteal region in a 33-year-old woman is presented. Immunohistochemical study and electron microscopic examination confirmed multidirectional differentiation of the tumor. The patient died 9 months after surgery, despite complementary multimodal therapy.

[Presence of lysozyme and alpha 1-antichymotrypsin in tumors of the fibrous histiocytoma type. Immunohistochemical evaluation of 52 cases].

In 52 cases of fibrohistiocytic sarcoma activity of alfa-1-antichymotripsin (ACT) in tumour cells was found in 47 cases and lysozyme in 16 cases. ACT was found in all types of tumour except angiomatous. Lysozyme was positive only in pleomorphic type, all these cases have also activity of ACT.