Bacterial colony size growth estimation by deep learning.

The bacterial growth rate is important for pathogenicity and food safety. Therefore, the study of bacterial growth rate over time can provide important data from a medical and veterinary point of view. We trained convolutional neural networks (CNNs) on manually annotated solid medium cultures to detect bacterial colonies as accurately as possible.

Annotated dataset for deep-learning-based bacterial colony detection.

Quantifying bacteria per unit mass or volume is a common task in various fields of microbiology (e.g., infectiology and food hygiene).

A glimpse of antimicrobial resistance gene diversity in kefir and yoghurt.

Antimicrobial resistance (AMR) is a global threat gaining more and more practical significance every year. The main determinants of AMR are the antimicrobial resistance genes (ARGs). Since bacteria can share genetic components via horizontal gene transfer, even non-pathogenic bacteria may provide ARG to any pathogens which they become physically close to (e.

Antimicrobial resistance genes in raw milk for human consumption.

The increasing prevalence of antimicrobial resistance (AMR) is a significant threat to global health. More and more multi-drug-resistant bacterial strains cause life-threatening infections and the death of thousands of people each year. Beyond disease control animals are often given antibiotics for growth promotion or increased feed efficiency, which further increase the chance of the development of multi-resistant strains.

Alteration of avian hepatic cytochrome P450 gene expression and activity by certain feed additives.

We investigated the effect of four feed additives, namely β-glucan, a drinking water acidifier (DWA), a sanguinarine-containing product (SN) and fulvic acid, on hepatic cytochrome P450 (CYP) mRNA expression and CYP enzyme activity in chickens. The test substances were given to the chickens in the recommended dose or in tenfold dose. The administration of 5 mg/kg body weight (bw) β-glucan and 0.

Alteration in Inflammatory Responses and Cytochrome P450 Expression of Porcine Jejunal Cells by Drinking Water Supplements.

The intestinal epithelium is the first determining barrier to the drugs administered . Cytochrome P450 (CYP) enzymes are substantial in the initial step of xenobiotic metabolism; therefore, intestinal CYP enzyme activities could be an important influencing factor of the oral utilization of xenobiotic substances. In this study, the effect of four drinking water supplements on CYP mRNA levels of porcine intestinal epithelial cells was examined.

Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR.

The rapid detection of Campylobacter spp. is of utmost importance for the reduction of infections in humans by contaminated food products. The standard culturing method (ISO 10272-1:2006) involves a high time and labour demand.

Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology.

Aims: To investigate whether the use of a novel synthetic medium in conjunction with impedimetric technology could provide a rapid and automated detection of Pseudomonas aeruginosa in water samples.

Methods And Results: A selective synthetic medium (Z-broth) in which the only carbon and nitrogen source is acetamide was applied in direct impedimetric examination for the selective isolation of P. aeruginosa.

Listeria monocytogenes food monitoring data and incidence of human listeriosis in Hungary, 2004.

Food samples were monitored for contamination with Listeria monocytogenes, and the incidence of human listeriosis was evaluated according to the data obtained in Hungary in the year 2004. Of the food samples tested, the bacterium was most often detectable in milk and dairy products, as 72.1% of all L.

A novel, selective synthetic acetamide containing culture medium for isolating Pseudomonas aeruginosa from milk.

A selective synthetic medium has been developed both in liquid (Z-broth) and solid (Z-agar) forms for selective isolation of Pseudomonas aeruginosa from foods. The simple, easy to prepare peptone-free synthetic medium contained acetamide that is metabolized to ammonia and acetic acid providing nitrogen and carbon supply. The medium contained no inhibitors.

Relationship of dietary fat and lysine level with body composition in broiler chickens.

The effect of dietary fat and/or lysine supplementation on the body composition of broiler chickens was studied. Body composition was measured by computed tomography (CT) and direct chemical analysis. Cockerel chicks from a previous experiment (Fekete et al.

Comparison of the protein composition of Erysipelothrix rhusiopathiae strains of subtype 1A isolated from ducks and pigs.

Erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (SDS-PAGE) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. To characterise the similarity of the strains, Pearson's correlation coefficient was calculated. The degree of similarity between duck strains originating from the same flock was 980-991, while that of strains isolated from ducks and pigs varied between 836 and 991 on a scale of 0 to 1000.

A synthetic, selective culture medium for Pseudomonas aeruginosa.

Selective and differentiating media consisting of simple chemical components have been developed, both in solid and in liquid form, for culturing Pseudomonas aeruginosa from foodstuffs. These media work on the basic principle that Gram-negative bacteria can utilize ammonia as inorganic nitrogen source. This principle has already been utilized for developing a culture medium for coliform bacteria (Szita and Biró, 1986; Szita et al.