Laparoscopic insulinoma enucleation for an uncinate lesion.

How I do laparoscopic insulinoma enucleation. This article discusses a laparoscopic approach for insulinoma enucleation, an approach which offers all the advantages of a laparoscopic approach and which can also be adapted to other pancreatic lesions requiring enucleation.

Evaluation of Patients with Vaccine Allergies Prior to mRNA-Based COVID-19 Vaccination.

During the initial rollout of coronavirus disease 2019 (COVID-19) vaccination in Singapore, the Ministry of Health (MOH) issued a recommendation that patients with a history of any previous vaccine allergy be referred to an allergist for further review of their suitability to proceed with mRNA-based COVID-19 vaccines. Patients fulfilling the above criterion were divided into three groups: immediate reaction (Group A), delayed reaction (Group B) and no/irrelevant reaction (Group C). They were subjected to either a skin prick test (SPT) and intradermal test (IDT) with polyethylene glycol (PEG) or polysorbate-containing products; direct injection with the Pfizer BNT162b2 vaccine in the allergy clinic; or injection at community vaccination centres, respectively.

Pseudo-Anaphylactic Reactions to Pfizer BNT162b2 Vaccine: Report of 3 Cases of Anaphylaxis Post Pfizer BNT162b2 Vaccination.

Anaphylactic reactions were observed after Singapore's national coronavirus disease 2019 (COVID-19) vaccination programme started in December 2020. We report the clinical and laboratory features of three patients in our institution who developed anaphylactic reactions after receiving the Pifzer BNT162b2 vaccine. IgM and IgG antibodies, but not IgE antibodies to the Pfizer BNT162b2 vaccine, were detected in all subjects.

Solvent-loaded metal-organic framework of type MIL-101(Cr)-NH for the dispersive solid-phase extraction and UHPLC-MS/MS analysis of herbicides from paddy field waters.

A novel solvent-loaded dispersive solid-phase extraction (SL-DSPE) method integrated with liquid-phase microextraction (LPME) has been developed by the direct loading of solvent into the pores of a metal-organic framework (MOF), MIL-101(Cr)-NH. Despite numerous advantages of MOFs, they are usually highly hydrophobic which limits their dispersibility and therefore effective contact with the analytes in aqueous samples. To overcome this and promote its interactions with polar compounds, MIL-101(Cr) was functionalized with -NH and loaded with a comparatively polar organic solvent, dichloromethane.

Fully automated graphitic carbon nitride-based disposable pipette extraction-gas chromatography-mass spectrometric analysis of six polychlorinated biphenyls in environmental waters.

A fully automated online emulsification-enhanced disposable pipette extraction-gas chromatography-mass spectrometry (EE-DPX-GC-MS) method has been developed for the extraction of six polychlorinated biphenyls (PCBs) from environmental waters. An in-house prepared material, graphitic carbon nitride (g-CN), was used as sorbent in a home-packed DPX device. The six PCBs studied include PCB 10, 28, 52, 153, 138 and 180.

Emulsification-assisted micro-solid-phase extraction using a metal-organic framework as sorbent for the liquid chromatography-tandem mass spectrometric analysis of polar herbicides from aqueous samples.

Two miniaturized sample preparation techniques, ultrasound-assisted emulsification microextraction (USAEME) and micro-solid-phase extraction (μ-SPE) have been integrated for the pre-concentration of four polar chlorophenoxy acid (CPA) herbicides from environmental aqueous samples. An metal-organic framework, MIL-101(Cr), characterized by its high porosity and large surface area, was explored as a sorbent. Despite the simplicity and convenience of μ-SPE, its use can potentially be limited by a long equilibration time, especially when applied to polar analytes.

Graphitic carbon nitride as sorbent for the emulsification-enhanced disposable pipette extraction of eight organochlorine pesticides prior to GC-MS analysis.

Graphitic carbon nitride (g-CN) was explored as a sorbent for the emulsification-enhanced (EE) disposable pipette extraction (DPX) of eight organochlorine pesticides (OCPs) from environmental waters. The OCPs, including α-hexachlorocyclohexane, Aldrin, α-Chlordane, Dieldrin, 4,4'-dichlorodiphenyldichloroethylene, 4,4'-dichlorodiphenyldichloroethane, Heptachlor and Heptachlor epoxide (Isomer A), were analyzed by gas chromatography-mass spectrometry. The sorbent g-CN was characterized by elemental analysis, X-ray diffraction, scanning electron microscopy, Fourier-transform infrared and Raman spectroscopy.

A hydrogel composite prepared from alginate, an amino-functionalized metal-organic framework of type MIL-101(Cr), and magnetite nanoparticles for magnetic solid-phase extraction and UHPLC-MS/MS analysis of polar chlorophenoxy acid herbicides.

A regenerable hydrogel composite is described that is comprised of alginate, amino-functionalized metal-organic framework (MIL-101(Cr)-NH) and magnetite nanoparticles. The composite was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, vibrating-sample magnetometry and Brunauer-Emmett-Teller measurement. The material was applied to the magnetic solid-phase extraction of six polar chlorophenoxy acid (CPA) herbicides.

A metal-organic framework of type MIL-101(Cr) for emulsification-assisted micro-solid-phase extraction prior to UHPLC-MS/MS analysis of polar estrogens.

A seamless two-step extraction procedure integrating ultrasound-assisted emulsification microextraction (USAEME) and vortex-assisted micro-solid-phase extraction (μ-SPE) was developed. A highly porous metal-organic framework of type MIL-101(Cr) is used as the sorbent, and ultra-high-performance liquid chromatography in combination with tandem mass spectrometry is used for detection. The steroid hormones estrone 17β-estradiol, estriol, and 17α-ethynylestradiol were extracted from water samples by using this method.

Cause and Clinical Presentation of Anaphylaxis in Singapore: From Infancy to Old Age.

Background: The study objective was to compare age-related differences in the cause and clinical presentation of anaphylaxis.

Methods: We conducted a prospective study of patients visiting the emergency department for anaphylaxis. Data were collected from 3 emergency departments from 1 April 2014 to 31 December 2015.

Method of quadriceps attachment following upper tibial resection.

Limb salvage procedures for osteosarcomas of the upper tibia present combined problems of knee-joint and tibial reconstruction. Many methods of overcoming this have been described. We describe a new technique of prosthetic knee-joint and upper-tibial reconstruction, combined with a vascularized fibular bone flap for reattachment of the quadriceps muscle and tendon unit, resulting in superior long-term function.