Can Shockwave Treatment Elicit a Molecular Response to Enhance Clinical Outcomes in Pressure Ulcers? The SHOck Waves in wouNds Project.

Wound healing requires the coordinated interaction of dermis cells, the proper deposition of extracellular matrix, re-epithelialization, and angiogenesis. Extracorporeal shock wave (ESW) is a promising therapeutic modality for chronic wounds. This study determined the biological mechanisms activated under ESW, facilitating the healing of pressure ulcers (PUs).

The Effect of Oleanolic Acid and Its Four New Semisynthetic Derivatives on Human MeWo and A375 Melanoma Cell Lines.

This study aimed to synthesize four new semisynthetic derivatives of natural oleanolic acid (OA) and, based on an analysis of their cytotoxic and anti-proliferative effects against human MeWo and A375 melanoma cell lines, select those with anti-cancer potential. We also screened the treatment time with the concentration of all four derivatives. We synthesized oxime 2 and performed its acylation with carboxylic acids into new derivatives and according to the methods previously described.

Modulating Properties of Piroxicam, Meloxicam and Oxicam Analogues against Macrophage-Associated Chemokines in Colorectal Cancer.

The mechanisms underlying the antineoplastic effects of oxicams have not been fully elucidated. We aimed to assess the effect of classic and novel oxicams on the expression/secretion of macrophage-associated chemokines (RTqPCR/Luminex xMAP) in colorectal adenocarcinoma cells, and on the expression of upstream the non-steroidal anti-inflammatory drug (NSAID)-activated genes , , , and as well as at the chemokine profiling in colorectal tumors. Meloxicam downregulated 9.

Heat Shock Proteins HSPA1 and HSP90AA1 Are Upregulated in Colorectal Polyps and Can Be Targeted in Cancer Cells by Anti-Inflammatory Oxicams with Arylpiperazine Pharmacophore and Benzoyl Moiety Substitutions at Thiazine Ring.

Heat shock proteins HSPA1/Hsp70α and HSP90AA1/Hsp90α are crucial for cancer growth but their expression pattern in colorectal polyps or whether they can be modulated by oxicams is unknown. We quantified (RTqPCR) and expression in 50 polyp-normal pairs in relation to polyp malignancy potential and examined the effect of piroxicam, meloxicam and five novel analogues on HSPA1 and HSP90AA1 expression (mRNA/protein) in colorectal adenocarcinoma lines. and were upregulated in polyps by 3- and 2.

The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells.

Molluscs are one of the sources of biologically active substances, which are now intensively studied, especially for their anti-cancer properties. Malignant melanoma originates from melanocytes, develops very quickly and is associated with poor prognosis. Therefore, the aim of the study was to assess the properties of biologically active compounds in sterile mucus isolated from slugs Limax maximus and Arion rufus.

L-Arginine/Nitric Oxide Pathway Is Altered in Colorectal Cancer and Can Be Modulated by Novel Derivatives from Oxicam Class of Non-Steroidal Anti-Inflammatory Drugs.

L-arginine/nitric oxide pathway metabolites are altered in colorectal cancer (CRC). We evaluated underlying changes in pathway enzymes in 55 paired tumor/tumor-adjacent samples and 20 normal mucosa using quantitative-PCR and assessed the impact of classic and novel oxicam analogues on enzyme expression and intracellular metabolite concentration (LC-MS/MS) in Caco-2, HCT116, and HT-29 cells. Compared to normal mucosa, , and were overexpressed in both tumor and tumor-adjacent tissue and solely in tumor-adjacent tissue.

The effect of 3-bromopyruvate on the properties of cathepsin B in the aspect of metastatic potential of colon cancer cells.

Background: Cathepsin B (CTSB, EC 3.4.22.

Interleukins 4 and 13 and Their Receptors Are Differently Expressed in Gastrointestinal Tract Cancers, Depending on the Anatomical Site and Disease Advancement, and Improve Colon Cancer Cell Viability and Motility.

Immunosuppressive interleukins (IL)-4 and 13 may directly promote cancer but neither their status nor role in gastrointestinal tract is clarified. We aim at quantifying ILs and their receptors in paired normal-tumor samples ( = 49/51) and sera ( = 263), using immunoassays and RTqPCR, and screening for their effect on colonic cancer cells. Both ILs were elevated locally at protein level in all cancers but only transcripts in colon were upregulated.

Transcriptional and Metabolomic Analysis of L-Arginine/Nitric Oxide Pathway in Inflammatory Bowel Disease and Its Association with Local Inflammatory and Angiogenic Response: Preliminary Findings.

L-arginine/nitric oxide pathway in Crohn's disease (CD) and ulcerative colitis (UC) is poorly investigated. The aim of current study is to quantify pathway serum metabolites in 52 CD (40 active), 48 UC (33 active), and 18 irritable bowel syndrome patients and 40 controls using mass spectrometry and at determining mRNA expression of pathway-associated enzymes in 91 bowel samples. Arginine and symmetric dimethylarginine decreased ( < 0.

3-Bromopyruvate as a potential pharmaceutical in the light of experimental data.

3-Bromopyruvate (3-BrPA) is an halogenated analogue of pyruvic acid known for over four decades as an alkylating agent reacting with thiol groups of many proteins. It enters animal cells like a lactate: via monocarboxylic acid transporters. Increasing interest in this compound, in recent times, is mainly due to hopes associated with its anticancer action.

Early induction of stress-associated Src activator/Homo sapiens chromosome 9 open reading frame 10 protein following photodynamic therapy.

Background: There are proteins, responsible for many basic cell functions (transmission of extracellular signals to cytoplasm or nucleus, cell growth, proliferation, migration, survival), which are activated and overexpressed in response to acute oxidative stress, especially tyrosine kinases. The oxidative stress-associated Src activator/Homo sapiens chromosome 9 open reading frame 10 protein (Ossa/C9orf10) protects cancer cells from oxidative stress-induced apoptosis by Src family kinases activation.

Methods: In this study precursor of protoporphyrin IX, 5-aminolevulinic acid and its encapsulated form were used in treating MCF-7 human breast cancer cells.

[Tuberculosis in Poland in 2007].

Unlabelled: In 2007 first time for many years registered small increase incidence of TB. In this year 8,616 tuberculosis cases notified it was higher--23 cases--than previous year. Incidence rate was 22.

[Tuberculosis in Poland in 2006].

Unlabelled: Tuberculosis has been a major health problem in Poland for many years. In 2006 y. decrease of tuberculosis notification rates in Poland has continued.

[Lung cancer in Poland in 1970-2004].

Introduction: The aim of this paper was to evaluate time trends of incidence and mortality from lung cancer by sex and age in Poland since 1970 to 2004.

Material And Methods: Basing on the data from National Cancer Registry - incidence and mortality due to lung cancer in Poland 2004 and time trends in the period 1970-2004 were analyzed.

Results: The were 20 359 cases of lung cancer in Poland in 2004: 15 741 cases in men (crude rate - 85,2/10(5)) and 4618 cases in women (crude rate - 23,8/10(5)).

[Tuberculosis in Poland in 2005].

Unlabelled: Tuberculosis has been a major health problem in Poland for many years. In 2005y. decrease of tuberculosis notification rates in Poland has continued.

[Tuberculosis in Poland in 2004].

Unlabelled: In 2004 y. decrease of tuberculosis notification rates in Poland has continued. In this year 9493 tuberculosis cases notified, incidence rate was 24.

[Cohort analysis for treatment outcomes TB pulmonary cases bacteriologically confirmed in Poland].

Unlabelled: In Poland since 1986 system of treatment outcomes monitoring (TOM) of all confirmed pulmonary TB (PTB) cases has been introduced. This system is based on the Central Tuberculosis Register TB detected and registered in given year from the whole network tuberculosis and lung diseases dispensaries in the country--/ 400 disp. in 2004 yr/.

[Incidence of tuberculosis in Poland 2004 and its trends].

Unlabelled: Tuberculosis has been a major public health problem in Poland for many decades. The aim of this investigation was to evaluate incidence of TB in Poland in 2004 and to examine trends of TB incidence in last decade. In 2004.

[Tuberculosis in Poland in 2003].

In 2003 year decrease of tuberculosis notification rates in Poland has continued. In this year 10 125 tuberculosis cases (TB) were notified in Poland--incidence rate--26.5 per 100 000 population, with the large differences in notification rates between voivodeships (from 16.

[Adverse events following immunization with BCG vaccine in Poland 1994-2000].

Background: In Poland, since 1955 BCG mass vaccinations have been compulsory. During the last two decades the vaccination program has been developed and includes: 1) primary vaccination of the newborn, 2) vaccination of children aged 7 and 12, and 3) revaccination of 18 year old adolescents. More than 95% of newborns and about 80% of older children of this population have been vaccinated.

[Tuberculosis in Poland 2000--at the turn of the century].

Unlabelled: The aim of this paper was to evaluate incidence, prevalence and mortality from TB in Poland in 2000 and to examine trends of TB incidence. Individual date on tuberculosis cases reported to National TB Register and data of prevalence and mortality from TB were analysed. The paper presents the epidemiological situation of TB in Poland in 2000.

[Safety of BCG vaccinations--adverse effects following immunization. Part II. Causes of complications. Clinical management].

Based on the review of Polish and world literature on adverse reactions after BCG immunization--causes of complications and clinical management has been described.

[Safety BCG vaccinations--adverse effects following immunization. Part I. Types, occurrence and registrations].

Based on review Polish and world literature on adverse reactions after BCG immunization--types, occurrence, recording and reporting system of complication has been described.