Ternary logic in the optical controlled-SWAP gate based on Laguerre-Gaussian modes of light.

The need set by a computational industry to increase processing power, while simultaneously reducing the energy consumption of data centers, became a challenge for modern computational systems. In this work, we propose an optical communication solution, that could serve as a building block for future computing systems, due to its versatility. The solution arises from Landauer's principle and utilizes reversible logic, manifested as an optical logical gate with structured light, here represented as Laguerre-Gaussian modes.

Analysis of the Possibility of Using Selected Tools and Algorithms in the Classification and Recognition of Type of Microstructure.

The aim of this research was to develop a solution based on existing methods and tools that would allow the automatic classification of selected images of cast iron microstructures. As part of the work, solutions based on artificial intelligence were tested and modified. Their task is to assign a specific class in the analyzed microstructure images.

Quantifying the quality of optical vortices by evaluating their intensity distributions.

Optical vortices are widely used in optics and photonics, impacting the measurements and conclusions derived from their use. Thus, it is crucial to evaluate optical vortices efficiently. This work aims to establish metrics for evaluating optical vortex quality to support the implementation procedure and, hence, provide a tool supporting research purposes and technological developments.

Optical vortex tracking algorithm based on the Laguerre-Gaussian transform.

Optical vortices are stable phase singularities, revealing a zero-point in the intensity distribution. The localization of this singular point is of significant importance for any application that relies on vortices and their behavior. However, there is still a need for an adaptable, fast, and precise method of singular point localization.

Broadband chromatic dispersion measurements in higher-order modes selectively excited in optical fibers using a spatial light modulator.

We propose an improvement of the interferometric method used up to now to measure the chromatic dispersion in single mode optical fibers, which enables dispersion measurements in higher-order modes over a wide spectral range. To selectively excite a specific mode, a spatial light modulator was used in the reflective configuration to generate an appropriate phase distribution across an input supercontinuum beam. We demonstrate the feasibility of the proposed approach using chromatic dispersion measurements of the six lowest order spatial modes supported by an optical fiber in the spectral range from 450 to 1600 nm.

Generation of light beams with custom orbital angular momentum and tunable transverse intensity symmetries.

We introduce a novel and simple modulation technique to tailor optical beams with a customized amount of orbital angular momentum (OAM). The technique is based on the modulation of the angular spectrum of a seed beam, which allows us to specify in an independent manner the value of OAM and the shape of the resulting beam transverse intensity. We experimentally demonstrate our method by arbitrarily shaping the radial and angular intensity distributions of Bessel and Laguerre-Gauss beams, while their OAM value remains constant.

Impact of fabrics from transgenic flax on cultures of skin cells.

Background: The development of a new type of wound dressing material that can support skin regeneration is an important challenge to improve treatment of chronic, non-healing wounds.

Objectives: The objective of this study was to compare the impact of flax fabrics from transgenic plants overexpressing phenolic acids and flavonoids (W92) and polyhydroxybutyrate (M48), as well as fabric from non-transgenic plant (Nike) on cultures of human skin cells.

Material And Methods: Flax fabric pieces as well as water extracts from the fabrics were co-cultured with human skin cells: keratinocytes, fibroblasts, dermal microvascular endothelial cells, and with monocytoid cell line (THP1) for 48 h.

Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts.

The goal of this study was to evaluate the most effective technique for extraction of phenolics present in flax shives and to assess their effect on human fibroblasts. Flax shives are by-products of fibre separation, but they were found to be a rich source of phenolic compounds and thus might have application potential. It was found that the optimal procedure for extraction of phenolics was hydrolysis enhanced by the ultrasound with NaOH for 24 h at 65°C and subsequent extraction with ethyl acetate.

V79 Fibroblasts Are Protected Against Reactive Oxygen Species by Flax Fabric.

Chinese hamster pulmonary fibroblasts (V79 cells) pre-treated with flax fabrics derived from non-modified or genetically engineered flax fibres and treated with HO revealed a markedly lower level of intracellular reactive oxygen species (ROS) than control, non-pre-treated cells. The fabrics were prepared from fibres derived from two kinds of transgenic plants: W92 plants, which overproduce flavonoids, and M type plants, which produce hydroxybutyrate polymer in their vascular bundles and thus in fibres. Incubating the V79 cells with the flax fabrics prior to HO treatment also reduced the amount of DNA damage, as established using the comet assay (also known as alkaline single-cell gel electrophoresis) and pulsed-field electrophoresis of intact cellular DNA.

Analytical model of the optical vortex microscope.

This paper presents an analytical model of the optical vortex scanning microscope. In this microscope the Gaussian beam with an embedded optical vortex is focused into the sample plane. Additionally, the optical vortex can be moved inside the beam, which allows fine scanning of the sample.

Transgenic potato plants with overexpression of dihydroflavonol reductase can serve as efficient nutrition sources.

Potato (Solanum tuberosum) is considered to be one of the most important crops cultivated in Europe and the entire world. The tubers of the potato are characterized by rich starch and protein contents and high concentrations of antioxidants, such as vitamin C and flavonoids. Notably, the presence of the phenolic antioxidants is of high importance as they have health-related properties.

Improved properties of micronized genetically modified flax fibers.

The aim of this study was to investigate the effect of micronization on the compound content, crystalline structure and physicochemical properties of fiber from genetically modified (GM) flax. The GM flax was transformed with three bacterial (Ralstonia eutropha) genes coding for enzymes of polyhydroxybutyrate (PHB) synthesis and under the control of the vascular bundle promoter. The modification resulted in fibers containing the 3-hydroxybutyrate polymer bound to cellulose via hydrogen and ester bonds and antioxidant compounds (phenolic acids, vanillin, vitexin, etc.

Preoperative ultrasonographic examination of the radial artery and the cephalic vein and risks of dialysis arterio-venous fistula dysfunction.

Background: Hemodialysis used as renal replacement therapy requires a well-functioning vascular access. Arterio-venous fistula (AVF) created on the forearm is the best vascular access, but it also reveals numerous complications such as: lack of fistula maturation and hemodynamically significant stenoses. Many risk factors of fistula dysfunction are still not identified.

Parvalbumin gene delivery improves diastolic function in the aged myocardium in vivo.

Abnormal relaxation of the heart, termed diastolic dysfunction, is a significant and growing problem that is a major cause of heart failure in the aged population. The potential of gene transfer of parvalbumin (Parv), a cytoplasmic calcium-binding protein, to improve diastolic function in the aged myocardium in vivo was evaluated. Despite evidence for an early developmental influence on the efficiency of Ad5 striated muscle transduction, results show that Ad5 gene transfer efficiency to adult cardiac myocytes in vitro is identical in young and old rats, suggesting that the basic processes of adenovirus binding and internalization are unaffected by aging.

Developmental and diel profiles of plasma corticosteroids in the bullfrog, Rana catesbeiana.

Corticosteroids synergize with the thyroid hormone (TH) at late metamorphic stages and might have a role in the hormonal regulation of amphibian metamorphosis. This role could be influenced by diel fluctuations, particularly if the peak of the plasma corticoids changed in relation to the TH peaks. Diel variation in plasma corticosteroids was studied in Rana catesbeiana prometamorphic and climax tadpoles on 18:6, 12:12 and 6:18 light:dark (LD) cycles.

Contribution of functional voltage-gated Na+ channel expression to cell behaviors involved in the metastatic cascade in rat prostate cancer: II. Secretory membrane activity.

The secretory membrane activities of two rat prostate cancer cell lines of markedly different metastatic potential, and corresponding electrophysiological characteristics, were studied in a comparative approach. In particular, voltage-gated Na(+) channels (VGSCs) were expressed in the strongly metastatic MAT-LyLu but not in the closely related, but weakly metastatic, AT-2 cells. Uptake and release of the non-cytotoxic marker horseradish peroxidase (HRP) were used as indices of general endocytotic and exocytotic membrane activity, respectively.

Targeting diastolic dysfunction by genetic engineering of calcium handling proteins.

Diastolic heart failure (HF) is associated with significant morbidity and mortality, and is a growing medical problem in this country. Diastolic dysfunction is defined as an abnormality in myocardial relaxation that impairs filling during diastole and contributes to the clinical syndrome of HF. Effective clinical strategies to treat diastolic dysfunction are limited.

Developmental and diel changes in plasma thyroxine and plasma and ocular melatonin in the larval and juvenile bullfrog, Rana catesbeiana.

Diel variation in plasma thyroxine (T(4)), and plasma and ocular melatonin was studied in Rana catesbeiana tadpoles and postmetamorphic froglets on 12:12 and 6:18 light/dark (LD) regimens. A progressive rise in plasma T(4) initiates metamorphosis while melatonin can modulate metamorphic progress. Changes in the phase of the rhythms of these two hormones during development might influence the hormonal regulation of metamorphosis.

Ionic and pharmacologic characteristics of epithelial cells in a semi-intact preparation of the rat ventral prostate gland.

Background: The essential ionic and pharmacologic characteristics of epithelial cells within the ducts of the prostate gland are not well known.

Methods: Experiments were carried out on segments of ventral prostate glands from adult male rats. By using sharp microelectrodes, intracellular epithelial cell and transepithelial (lumen) potentials were recorded in response to ionic substitution and application of ion channel blockers, hormones, and other pharmacologic agents related to prostatic function.

In vivo acceleration of heart relaxation performance by parvalbumin gene delivery.

Defective cardiac muscle relaxation plays a causal role in heart failure. Shown here is the new in vivo application of parvalbumin, a calcium-binding protein that facilitates ultrafast relaxation of specialized skeletal muscles. Parvalbumin is not naturally expressed in the heart.

Electrophysiological recordings from the rat prostate gland in vitro: identified single-cell and transepithelial (lumen) potentials.

Objective: To develop a preparation for the in vitro maintenance of the rat prostate gland and thus allow intracellular and transepithelial voltage measurements.

Materials And Methods: Ventral prostate glands from male rats were dissected free of connective tissue, separated into smaller lobes and maintained in vitro at 30 degrees C. Voltages were recorded with sharp micropipettes in identified cellular and luminal compartments, differentiated by several electrophysiological and histological parameters, including intracellular staining.

Neuronal excitability and conduction velocity changes in hippocampal slices from streptozotocin-treated diabetic rats.

The effects of streptozotocin-induced diabetes on the electrophysiological properties of central neurones were investigated in rat hippocampal slices. No differences in membrane potential, input resistance or action potential parameters could be detected in pyramidal or granule cells. However, a slowing of conduction velocity in granule cells and an increase in excitability of fibre volleys in both the perforant pathway and granule cell mossy fibre projections was observed.