Construction of SHERLOCK-based sgRNA for SARS-CoV-2 Diagnostics from Indonesia.

Handling a pandemic requires high sensitivity, high specificity, simple, fast, and flexible tests. How- ever, conventional test methods (RT-PCR and Rapid Antigen) have weaknesses in test efficiency. Specific High sensitivity Enzymatic Reporter un-LOCKing (SHERLOCK), is a new technology that can detect nucleic acids even with limited sample preparation, but with high sensitivity, high spec- ificity, rapidly, and flexibly.

Redesigning Primer of ITS2 (Internal Transcribed Spacer 2) for Specific Molecular Characterization of Malaria Vectors Anopheles Species.

Background: mosquitoes are vectors of malaria, which is a serious health issue in Indonesia. Thus, vector control is an important approach taken to overcome this disease. The first and most important step in vector control is vector identification.