[Effect of intraoperative lavage of the large intestine with hyperoxygenated solution on the healing of large intestinal anastomosis].

In the experiment on 80 rats, the intraoperative lavage of the colon (IL) in its perforation and acute obstructive ileus was performed. Lavage was performed before wound closure and placing the anastomosis. In using IL with hyperoxigenated solution, the favourable course of the postoperative period, increase in number of the animals survived, decrease in incidence of complications were noted.

[Perivascular lymphoid nodules].

As a result of investigation of lymphoid formations in the walls of the human tracheobronchial tree and the pulmonary stroma by means of morphological and morphometrical techniques, certain regularity in arrangement of the perivascular lymphoid noduli has been stated along the course of the lympho- and hemomicrocirculatory bed links. The periarterial lymphoid noduli of the tracheobronchial tree have certain morphological similarity and some differences, when comparing them with usual lymphoid noduli.

[The morphofunctional changes in the perivascular and lymphoepithelial nodes of the respiratory organs following antigenic stimulation].

Morphological, experimental-immunological methods with mathematical analysis were used in 108 Wistar line rats in the pre- and postnatal ontogenesis to study the lymphoid structures of the trachea, bronchi and stroma of the lungs. Two types of lymphoid nodules were detected in the respiratory organs of rats and man: 1. perivascularly located in the wall of the trachea, bronchi and stroma of the lungs; 2.

[The action of phage 52A on energy processes in Staphylococcus aureus].

Phage infection leads to the dissipation of the transmembrane difference of electric potentials, measured by the adsorption of lipophilic permeant ions of tetraphenyl phosphonium, in staphylococci. Besides, the reversible stimulation of cell respiration processes is observed. The duration of these effects is comparable with the period of the injection of phage DNA, equal to 2-3 minutes.

[The age-related characteristics of the lymphoid structures in the human respiratory organs].

It was found that the wall of the tracheo-bronchial tree and the lung stroma of man has two types of nodules: those located in the mucous membrane and adjoining the epithelium and those located in the fibrous-cartilagenous part of the wall of the trachea, bronchi and stroma of the lungs. The latter serve the purpose of defense of the internal medium of the body. It was established that lymphoid nodules of the respiratory organs undergo age-related involution.

[The effect of valinomycin and nigericin on the efficacy of bacteriophage infection of staphylococcal cells].

The effect of ionophore antibiotics, valinomycin and nigericin, on the generation of the membrane potential, the pH gradient and the efficacy of phage infection in tetracycline-resistant staphylococci has been studied. Valinomycin at a concentration of 0.5 microM induces the dissipation of the membrane potential, and nigericin at a concentration of 12.

[The enzyme activity of energy metabolism in antibiotic-resistant staphylococci].

The rates of lactate accumulation and glucose oxidation by the clinic strains of Staphylococcus aureus were determined. The inhibition grade of the glucose oxidation by cyanide indicates that respiratory chain of staphylococci participates in the oxidizing processes. During glucose oxidation the respiratory chain generates the difference of electric potentials -156 divided by -179 mV.

[Experimental characteristics of the immune response of lymphoid organs to fungal and staphylococcal antigens].

Increased proliferative and functional activities of the lymphoid organs and a high mitotic activity of the lymphocyte blast forms were recorded by autohistoradiography (3H thymidine and 35S-labeled sulfate) in 60 guinea pigs in acute experiments (a challenge with staphylococcal or fungal antigen). Degeneration of the lymphoid tissue and substitution of the lymphoid tissue with fatty tissue were observed in a chronic experiment. Discoordination of the immune response consisted in depression of the T-component, with the B-component retaining high activity; these shifts were the most manifest after challenge with fungal antigen.

[Magnitude of the proton moving force of Staphylococcus aureus cells and features of the interaction of staphylococci with phages].

The magnitude of the transmembrane electrical potential difference and the proton gradient across the energy-transducing membrane of Staphylococcus aureus were determined. The delta psi value was shown to rise from 100 to 160 mV upon alkalinization of the medium within the pH range of 5.0-8.

[Energy-dependent transport of tetraphenylphosphonium ions in Staphylococcus aureus].

Transmembrane potential differences on the membrane of staphylococci were measured using tetraphenylphosphonium selective electrodes. When rising pH from 6.0 to 8.

[Duration of the mitotic cycle periods of lymphoid cells from the respiratory organs and the effect of the intranasal administration of gamma-globulin].

Parameter studies of mitotic cycles of the lymphoid population in the rats respiratory system showed that after gamma-globulin injection to the respiratory system the main mechanism of cellular division consists of synchronized entry of cells to the mitotic cycle, shortening of the parameters and time of cellular regeneration.

[Effect of intratracheal gamma-globulin administration on the mitotic activity and proliferative pool of lymphoid cells of the respiratory tract].

After injection of gamma-globulin into respiratory tracts of rats a proliferative pool of lymphoid cells in the lungs increases and remains unchanged in the spleen. The increase in the proliferative pool was accompanied by a decrease in a mitotic cycle in the S-period. Immunization was followed by intensification of division processes in a group of medium lymphocytes and processes of blast cells differentiation.

[Morphofunctional changes in the lymphoid tissue of the respiratory organs in gamma-globulin administration].

By means of macro-microscopic, histologic, radioautographic and immunomorphologic methods, three forms of the lymphoid tissue structural organization have been revealed in the rat respiratory organs: a lymphoid cluster, a lymphoid follicle and a lymphoepithelial nodule. After gamma-globulin is administered intranasally, the ratio of the lymphoid cells in the organs mentioned changes: the number of small lymphocytes decreases, while that of middle, large lymphocytes and blast cells increase. The lymphoid follicles consist of a central and a marginal zones, in the lymphoepithelial nodules the subepithelial and paranodular zones also exist.

[Morphofunctional changes in the glands of the tracheobronchial system following gamma globulin administration].

Morpho-functional changes and synthesis of carbohydrate compounds in the mucous membrane of the rat and cat trachea and bronchi have been studied 2, 4, 7, 14 and 24 days after a single and twice administration of gamma-globulin into the respiratory tract and after repeated intrapleural and intramuscular administration following sensibilization with a complete Freund's adjuvant. Dynamic of the changes observed depends, to a great extent, on the mode and multiplicity of administration of the adjuvant. When gamma-globulin is administered into the respiratory tract or intrapleurally, a more active incorporation of 3H-glucose and 35S-sodium sulfate into chondroitinsulphate A, C, sialic acids and glycogen, which are synthesized by cells of the tracheobronchial system glands, occurs.

[Radioautographic study of acid glycoprotein synthesis by tracheal and bronchial glands during ontogeny].

The synthesis of acid glycoproteins in the glands of the respiratory pathway has been studied radioautographically by means of sodium sulfate in the cat ontogenesis. 35S-sulfate incorporation into the fetal gland has been stated to increase by the time of delivery. After birth and in young kittens, 35S-sulfate incorporation into the gland decreases.

[Histoautoradiographic study of sulfated mucopolysaccharides in glandular epithelial cells of the lower respiratory passages].

It is established that mucous and serous cells of trachea and bronchia uptake radio-active sulphate with different rate. The intensity of radioactive sulphate incorporation by the mucous cells is higher than by the serous ones. The greater intensity of the incorporation is observed 12-24 hours after isotope's injection.