Publications by authors named "Sylwia Lapinska-Szumczyk"

Article Synopsis
  • Liquid biopsies, especially focusing on circulating tumor cells (CTCs) in blood, provide crucial insights into cancer spread, but current analysis is mainly limited to counting and basic marker profiling.
  • A study analyzed CTCs' size, shape, and features from different cancers using advanced imaging methods, finding notable size and shape differences among at least four different cell types.
  • Findings revealed that lung cancer produced the largest CTCs, while prostate and breast cancers had smaller ones, and distinct characteristics in cell shape corresponded to their epithelial or mesenchymal origins.
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Article Synopsis
  • Platelets can be influenced by cancer through a process called education, leading to a specific transcriptional profile that can aid in cancer detection, particularly for ovarian cancer.
  • A study involving 761 patients with confirmed adnexal masses and 167 healthy controls across multiple countries showed that tumor-educated platelets (TEPs) had a high diagnostic accuracy, with AUCs (area under the curve) ranging from 0.887 to 0.923 in validation cohorts.
  • Combining TEPs with the CA125 biomarker further improved detection accuracy, and TEPs showed potential across diverse populations and cancer stages, but further extensive studies are necessary before they can be widely used in clinical settings.
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Article Synopsis
  • Early detection of tumors in cancer patients leads to better treatment outcomes for less advanced cancers.
  • Tumor-educated platelets (TEPs) can be used for cancer detection via RNA-based blood tests, identifying 18 different cancer types with high accuracy.
  • The thromboSeq test showed 99% specificity in asymptomatic controls, accurately detecting two-thirds of cancers in advanced stages, and helped determine the origin of tumors in over 80% of cases.
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Background: Liquid biopsy is a minimally invasive collection of a patient body fluid sample. In oncology, they offer several advantages compared to traditional tissue biopsies. However, the potential of this method in endometrial cancer (EC) remains poorly explored.

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Whilst the role of eukaryotic translation initiation factors (eIFs) has already been investigated in several human cancers, their role in endometrial cancer (EC) is relatively unknown. In the present retrospective study, 279 patients with EC (1180 samples) were included (mean age: 63.0 years, mean follow-up: 6.

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Four molecular subtypes have lately been established in endometrial cancer basing on estrogen receptor (ER), progesterone receptor (PR) and HER2 status: ER+/PR+/HER2+, ER+/PR+/HER2-, ER-/PR-/HER2+ and ER-/PR-/HER2-. The subtypes have shown diversity in terms of prognosis, clinicopathological and molecular characteristics, with ER+/PR+/HER2- and ER-/PR-/HER2+ group exhibiting exceptionally benign and aggressive behavior, respectively. We have further characterized the subtypes in the context of pathways known to drive endometrial carcinogenesis: phosphatidylinositol 3-kinase (PI3K)-AKT pathway (ERBB/PI3K pathway), TP53 system, and the mismatch repair (MMR) mechanism.

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Background: Endometrial cancer (EC) is a hormone-related disease, showing highly diverse features of ER/PR/HER2 status-based molecular subtypes. Long noncoding RNA (lncRNA) HOX antisense intergenic RNA (HOTAIR) has recently emerged as a key molecule in many cancers, triggering epithelial-mesenchymal transition (EMT)-mediated cancer stem cell (CSC) formation, but little is known about its significance in EC. Thus, we aimed to investigate the clinical significance of HOTAIR itself in different molecular subtypes of EC and possible links between HOTAIR, EMT and CSC-related markers.

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Objectives: The aim the study was to compare two groups of endometrial cancer patients (below and above 45 years of age) in the aspect of clinicopathological and molecular data.

Material And Methods: The study encompassed 456 primary tumour samples retrospectively collected from a cohort of endometrial cancer patients, primarily treated by surgery Molecular analysis covered: copy number variations of 10 genes (TOP2A, ERBB1, ERBB2, ERBB3, ERBB4, MYC, CCND1, ESR1, PIK3CA, RAD21) analyzed by quantitative PCR; mRNA expression of 6 genes (SCGB2A2, RAD27, RUNX1, SNAI1, SNAI2, PROM1) analyzed with the use of reverse transcription quantitative PCR; protein expression analysis of 8 markers (PGR, ESR1; ERBB1, ERBB2, ERBB3, ERBB4, TOP2A, pAKT1) performed with the use of immunohistochemistry.

Results: The younger group of patients was characterized by less frequent hypertension (p <0.

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Endometrial cancer (EC) is a hormone-dependent, most frequent malignancy of the female genital tract, yet no molecular subtype classification based receptor status (estrogen receptor [ER], progesterone receptor [PR], human epidermal growth factor receptor 2 [HER2]) has been established so far. Assuming that molecular subtypes might differ fundamentally in EC, we analyzed expression levels of ER, PR, and HER2 with immunohistochemistry and aimed to determine clinical significance of four molecular subtypes: ER+/PR+/HER2+; ER+/PR+/HER2-, ER-/PR-/HER2+, and ER-/PR-/HER2-. The study included 400 formalin-fixed paraffin-embedded primary tumor EC samples which covered all stages of endometrial carcinoma, from IA to IVB.

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Intratumor heterogeneity implies heterogeneous protein function, facilitating tumor adaptation which results in therapeutic failure. We hypothesized that tumor heterogeneity at protein level may influence the course of the disease. As a single biopsy might not represent the full biologic complexity of the tumor, we have analyzed immunohistochemically four different cores obtained from each primary tumor within the cohort of 364 patients with endometrial cancer (EC).

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The molecular background of endometrial cancer (EC) has not been fully elucidated. In the present study, we developed a quantitative PCR (qPCR) platform to examine the gene dosages of the potential molecular markers MGB1, TOP2A, ERBB1-4, MYC, CCND1, ESR1 and PI3K. The platform was applied in samples collected from 157 EC patients (stage I-IV) to verify its clinical utility and to examine the diagnostic and prognostic significance of the analysed biomarkers.

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Background/aim: Epithelial-mesenchymal transition (EMT) and cancer stem cells (CSCs) are presumed to be key conditions for malignancy. Data concerning their role in endometrial cancer (EC) are scarce. We aimed to investigate the possible link between EMT and CSCs markers in EC samples.

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Objectives: The aim of the study was to compare the results of single-photon emission computed tomography-computed tomography (SPECT-CT) with those of intraoperative gamma probe detection and assess the clinical utility of SPECT-CT for sentinel lymph node biopsy in endometrial cancer.

Materials And Methods: We investigated 70 patients with endometrial cancer who underwent total abdominal hysterectomy, bilateral salpingo-oophorectomy, and sentinel lymph node biopsy (routine pelvic and para-aortic lymphadenectomy was additionally performed in high-risk patients). Tc-99m radiocolloid albumin was injected into the cervix and a blue dye was injected superficially into the fundus.

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Cohesins and cohesin-regulated genes are deregulated in numerous types of human cancer. However, data concerning their status and role in endometrial cancer are scarce. This study aimed to determine the clinical significance of double-strand-break repair protein rad21 homolog (RAD21) and runt-related transcription factor 1 (RUNX1) gene dosage and mRNA expression in endometrial cancer.

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The HtrA family of serine proteases takes part in cellular stress response including heat shock, inflammation and cancer. Downregulation of human HtrA1 and HtrA3 genes has been reported in some cancers, including endometrial cancer (EC), suggesting a tumor-suppressor role for both genes. The mechanism of the HtrA function is not known, however, evidence exists showing that both HtrA1 and HtrA3 regulate biological processes by modulating TGF-beta signaling.

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Objectives: The purpose of the research was to analyze the frequency and location of endometrial cancer recurrence in a group of women who underwent surgery due to neoplasm and frequency of relaparotomy because of the neoplasm recurrence.

Materials And Methods: 292 women underwent operation in Dept. of Gynecology at Medical University of Gdansk due to endometrial cancer in clinical stage IA-IV by FIGO 1988 in the years of 1981-1996.

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Clear statement that pediatric neoplasms are really rare is not easy. Thus the incidence of rare tumours in children has not been defined so far. The paper efforts to assess the topic of rare tumours of childhood in the Polish population.

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Objectives: The aim of the study was to assess procreation in group of patients who were treated by conservative operation for borderline tumors of the ovary.

Design: The analysis included 42 patients conservatively operated for ovarian tumor of borderline malignancy in Department of Gynecology Medical University of Gdansk between 1978-2000. The incidence of pregnancy, age of patients, tumor pathology, type of conservative surgery and the course of pregnancy and labour were evaluated in this study.

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Objectives: The aim of the study was to determinate the frequency of obesity, hypertension and diabetes mellitus and its potential influence on clinical stage of endometrial cancer in a large, representative group of 292 patients operated because of endometrial cancer.

Materials And Methods: The group of 292 women operated because of endometrial cancer in clinical stage IA-IV between 1981-1996 in 2nd. Dept.

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Objectives: The aim of the study was the clinical analysis of value of pelvic lymphadenectomy in surgical treatment of endometrial carcinoma.

Material And Methods: The group of 420 women treated for of endometrial cancer in stage IA-IV by FIGO 1988 was analysed. All these women were operated in 2nd Dept.

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A case of rupture of myomatous uterus during term delivery was described. 38-years old women delivered in 41 Hbd of fourth pregnancy. She was after two normal deliveries and one abortion.

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