Publications by authors named "Sylvie Seguin"
Rhizophagus irregularis is the model species for arbuscular mycorrhizal fungi (AMF) research and the most widely propagated species for commercial plant biostimulants. Using asymbiotic and symbiotic cultivation systems initiated from single spores, advanced microscopy, Sanger sequencing of the glomalin gene, and PacBio sequencing of the partial 45S rRNA gene, we show that four strains of R. irregularis produce spores of two distinct morphotypes, one corresponding to the morphotype described in the R.
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- - Arbuscular mycorrhizal fungi (AMF) form symbiotic relationships with most land plants, but coding information is mostly available only for a few well-studied species like Rhizoglomus and Gigaspora.
- - This study presents large-scale transcriptome data from eight lesser-known AMF species, revealing their functional diversity and confirming evolutionary connections among them.
- - The research shows that RNA-seq from low-input RNA is as reliable as traditional methods, paving the way for better understanding of fungal functions and phylogenetic relationships with minimal RNA samples.
The use of microwave irradiation as a source of energy to clear and stain intra-radical arbuscular mycorrhizal fungi propagules has been tested on a variety of indigenous and cultivated herbaceous plants. The aim of the study was to evaluate the efficiency of microwave irradiation on root softening, fungi tissue staining, and preservation of DNA integrity for subsequent molecular analyses. The proposed methodology has been adapted from the standard procedures used to detect and quantify mycorrhizal root colonization levels.
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