Novel imprinted transcripts from the Dlk1-Gtl2 intergenic region, Mico1 and Mico1os, show circadian oscillations.

Most of the known imprinted genes are assembled into clusters that share common imprinting control regions (ICRs). Non-coding transcripts are often associated with ICRs and implicated in imprinting regulation. We undertook a systematic search for transcripts originating from the Dlk1-Gtl2 intergenic region that contains the ICR for the chromosome 12 imprinted cluster and identified two overlapping transcripts expressed from opposite strands exclusively from the maternal chromosome.

Screening for pain phenotypes: analysis of three congenic mouse strains on a battery of nine nociceptive assays.

In an attempt to identify new genes responsible for variability in pain sensitivity, we tested three congenic mouse strains--in which a small portion of the genome of the MOLF/Ei strain has been placed on a C57BL/6 genetic background--on a battery of nine nociceptive assays, chosen to reflect those assays in most common use in the pain literature. Mice of both sexes were evaluated by two different testers at different points in time, allowing us to examine the relative importance of genotype, sex, tester and cohort effects on data from these assays. We find strong evidence for the existence of two quantitative trait loci (i.

Increased plasticity of genomic imprinting of Dlk1 in brain is due to genetic and epigenetic factors.

The expression of six imprinted genes (Dlk1, Gt12, Igf2r, Kcnq1, Nnat, and Peg1) was examined in brains of 21 mice derived from N2 x N2 intercrosses between C57BL/6 and MOLF/Ei strains. Imprinting of Igf2r, Kcnq1, Gt12, and Dlk1 varied among individuals. As three of these genes are implicated in cell-cell signaling or cell-environment interactions, variation in their imprinting may influence a wide range of biological processes from cell differentiation to behavior.

Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos.

The distal part of the mouse Chr 12 contains a cluster of reciprocally imprinted genes. Recently we found a grandparental origin-dependent, transmission-ratio distortion (TRD) in this region. The TRD resulted from postimplantation loss of embryos that inherited the distal Chr 12 alleles from the maternal grandfather.

Inheritance patterns of maternal alleles in imprinted regions of the mouse genome at different stages of development.

Deviations from Mendelian 1:1 transmission ratio have been observed in mice and humans. With few exceptions, the mechanism leading to transmission-ratio distortion (TRD) remains obscure. We proposed that a genomic imprinting mechanism plays a key role in the genesis of grandparental origin-dependent TRD (Naumova et al.

Transforming growth factors-α and -β expression in fertilized and parthenogenetic pre-implantation mouse embryos: RNA detection with fluorescent in situ hybridization.

Transforming growth factor (TGF)-α and -β are expressed in pre-implantation embryos and could play an important role in development. Usually, gene expression is studied using reverse transcription-polymerase chain reaction (RT-PCR) but here, we developed a fluorescent in situ hybridization (FISH) procedure applied on whole embryos that were spread and fixed on slides after hypotonic shock. This procedure allowed a rapid and specific detection of mRNA.