Comparative genomic hybridization analysis of sperm DNA apoptosis after exposure to heat shock.

Purpose: A DNA disc chip assay based on comparative genomic hybridization (CGH) was developed to measure sperm DNA integrity. The objective was to correlate DNA integrity of heat-treated sperm with the sperm capacitation index (CI) determined from the sperm penetration assay.

Methods: Basic semen and kinematic parameters were measured (N = 6).

A simple DNA disc chip in a microarray design based on modified comparative genomic hybridization for sperm DNA analysis.

Objective: A DNA disc chip assay, based on comparative genomic hybridization, was designed to measure changes in sperm DNA intensities. The objective was to analyze the DNA integrity of hyperactive sperm cells after mild heat treatment.

Design: The assay based on a multiple cell comet assay was used to analyze changes in genomic DNA.