Publications by authors named "Sylvain Comte"
Protein adsorption onto capillary wall often hampers CE separations, particularly in the CZE mode. Electrostatic interactions are not the only factors affecting adsorption, as hydrophobic interactions and/or protein conformational changes are also involved in the adsorption phenomenon. Numerous methods can be used to reduce or avoid adsorption, such as (i) addition of low molecular weight molecules in the BGE, (ii) use of surfactants, or (iii) capillary coatings.
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- The study analyzed the molecular epidemiology of 50 infectious bursal disease virus (IBDV) strains from 17 countries between 1972 and 2002, focusing on their genome segments A and B.
- Results showed that while most strains exhibited co-evolution in genome segments, 26% had different phylogenetic relationships based on the segment analyzed, suggesting possible natural reassortment.
- An identified reassortant strain displayed reduced pathogenicity compared to typical virulent strains, but the reasons for this lowered pathogenicity are still unclear, as no significant differences were found in bursal lesions or immune responses in infected chickens.
The 99323 Egyptian isolate of infectious bursal disease (IBD) virus (IBDV) was identified during an international survey of acute IBD cases. Its unique antigenicity was characterized by a markedly reduced binding of neutralizing monoclonal antibodies 3, 4, 5, 6, 8 and 9 in an antigen-capture enzyme-linked immunosorbent assay. Nucleotide sequencing of the genome region encoding the VP2 major immunogenic domain in 99323 revealed amino acid changes occurring at positions critical for antigenicity, but phylogenetic analysis demonstrated that 99323 was related to typical, very virulent IBDV (e.
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