Prime editing is a versatile CRISPR/Cas-based precise genome-editing technique for crop breeding. Four new types of prime editors (PEs) named PE6a-d were recently generated using evolved and engineered reverse transcriptase (RT) variants from three different sources. In this study, we tested the editing efficiencies of four PE6 variants and two additional PE6 constructs with double-RT modules in transgenic rice (Oryza sativa) plants.
View Article and Find Full Text PDFThe role of the most fungal endophytes in the host plant growth and production of metabolites under stress conditions is still unknown. Fungal endophytes occur in almost all plants to benefit the host plants exposed to biotic and abiotic stress. In the present work, we investigated salt (NaCl) stress alleviation capability of a fungal endophyte (Porostereum spadiceum-AGH786).
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