Stability indicating RP-HPLC method for simultaneous determination of piroxicam and ofloxacin in binary combination.

A simple and precise RP-HPLC method was developed for simultaneous determination of piroxicam and ofloxacin in pharmaceutical formulations and human serum. Optimum separations of piroxicam, ofloxacin and stress-induced degradation products were achieved by use of Hypersil BDS C8 column (250 x 4.6mm, 5 μm).

Stability indicating HPLC method for the simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations.

Background: A simple, specific, and fast stability indicating reverse phase liquid chromatographic method was established for instantaneous determination of moxifloxacin and prednisolone in bulk drugs and pharmaceutical formulations.

Results: Optimum chromatographic separations among the moxifloxacin, prednisolone and stress-induced degradation products were achieved within 10 minutes by use of BDS Hypersil C8 column (250 X 4.6 mm, 5 μm) as stationary phase with mobile phase consisted of a mixture of phosphate buffer (18 mM) containing 0.

Lamotriginium dihydrogen phosphate-4-(dimethyl-amino)-benzaldehyde (1/1).

In the title compound, C(9)H(8)Cl(2)N(5) (+)·H(2)PO(4) (-)·C(9)H(11)NO [systematic name: 3,5-diamino-6-(2,3-dichloro-phen-yl)-1,2,4-triazin-2-ium dihydrogen phosphate-4-(dimethyl-amino)-benz-alde-hyde (1/1)], inter-molecular N-H⋯O and O-H⋯O hydrogen bonds produce R(2) (2)(8) and R(3) (2)(8) rings, generating a layer. Inter-molecular N-H⋯N inter-actions also occur. The dihedral angle between the rings in the cation is 71.

[1-(Carboxy-meth-yl)cyclo-hexyl]-methan-aminium dihydrogen phosphate.

In the title salt, C(9)H(18)NO(2) (+)·H(2)PO(4) (-), the cyclo-hexane ring is puckered, the total puckering amplitude Q(T) being 0.555 (4) Å, and an intra-molecular N-H⋯O hydrogen bond generates an S(7) ring. In the crystal structure, inter-molecular N-H⋯O and O-H⋯O hydrogen bonds lead to R(2) (2)(14), R(3) (3)(8) and R(4) (2)(8) rings, generating a two-dimensional layer.