Evaluation of QT parameters in patients of thalassaemia major with iron overload.

Objective: To study QT parameters in Thalassaemia Major patients and their association with serum ferritin levels.

Methods: This cross-sectional study was conducted at Ziauddin University, Karachi, from February 2013 to September 2014, and comprised patients of Thalassaemia Major. Participants' serum ferritin levels were determined and electrocardiography was performed.

Liquid chromatography tandem mass spectrometry method for the simultaneous stereoselective determination of venlafaxine and its major metabolite, O-desmethylvenlafaxine, in human plasma.

A sensitive, accurate and highly stereoselective assay for the simultaneous determination of venlafaxine (VEN) and its equipotent metabolite, O-desmethyl venlafaxine (ODV), in human plasma was developed and validated. Analytes were simultaneously extracted from plasma using solid-phase extraction and detected by tandem mass spectrometry in positive ion mode with a turbo ion spray interface. Deuterium-labeled VEN and ODV were used as internal standards.

The intercept and slope of breathlessness/chest pain-heart rate relationship in patients with coronary artery disease using exercise tolerance test.

The most common cause of morbidity and mortality all over the world is Coronary artery disease. The traditional risk factors for Coronary artery disease are hypertension, diabetes mellitus, family history, smoking, dyslipidaemia and obesity. Chest pain and dyspnoea are the two common complaints of patients with Coronary artery disease.

The relationship between exertional chest pain/dyspnoea--heart rate in patients with coronary artery disease using Exercise ToleranceTest.

Objectives: To observe the relationship between chest pain/dyspnoea-heart rate during exertion in patients with angiographically proved Coronary artery disease compared to patients having negative Exercise Tolerance Test in a private and public sector hospital.

Methods: This is an observational two centre study from Karachi in which 150 patients referred for Exercise ToleranceTest (ETT) at Ziauddin University Hospital, Clifton campus and National Institute of Cardiovascular Diseases Karachi were included. The period of study extended from September 2008 to March 2010.