Publications by authors named "Sydwell P Maputla"
Development and validation of an LC-MS/MS method for quantifying total and unbound doravirine in human plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Article Synopsis
- A highly sensitive LC-MS/MS method was developed to measure total and unbound doravirine in plasma from patients in a clinical trial, utilizing a single calibration curve for both forms of the drug.
- The method involved protein precipitation for total doravirine extraction and ultrafiltration for unbound doravirine, employing specific chromatography techniques to ensure accurate analysis.
- Validation tests confirmed the method's precision and robustness against various sample conditions, meeting international standards for effective quantification of doravirine concentrations.
For the effective treatment of tuberculosis with first-line anti-tubercular drugs, drug concentrations need to be measured at the site of infection to determine drug exposure. To enable the measurement of the anti-tuberculosis drugs isoniazid and pyrazinamide in the nervous system of patients with tuberculous meningitis, an analytical method was developed and validated for the quantification of these drugs in human cerebrospinal fluid. Samples were prepared by solid phase extraction using Strata-X polymeric extraction plates.
View Article and Find Full Text PDFIntroduction: Isoniazid (INH) is one of the most effective and potent first-line anti-tubercular drug. INH is also effectively administered as a preventative monotherapy and has been shown to significantly reduce TB incidence. INH is primarily metabolised to acetyl-isoniazid (AcINH) in the liver.
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