Buffer Effects in Zirconium-Based UiO Metal-Organic Frameworks (MOFs) That Influence Enzyme Immobilization and Catalytic Activity in Enzyme/MOF Biocatalysts.

Novel biocatalysts that feature enzymes immobilized onto solid supports have recently become a major research focus in an effort to create more sustainable and greener chemistries in catalysis. Many of these novel biocatalyst systems feature enzymes immobilized onto metal-organic frameworks (MOFs), which have been shown to increase enzyme activity, stability, and recyclability in industrial processes. While the strategies used for immobilizing enzymes onto MOFs can vary, the conditions always require a buffer to maintain the functionality of the enzymes during immobilization.