Publications by authors named "Sydney E Gies"
Optimized protocol for the multiomics processing of cryopreserved human kidney tissue.
Am J Physiol Renal Physiol
November 2024
Article Synopsis
- The study talks about collecting kidney tissue samples from patients to use them for research and understanding diseases better.
- They found a way to keep these tissues preserved without needing super-cold liquid nitrogen, which makes it easier for doctors to use them in their work.
- By testing these samples with advanced technology, they hope to identify new ways to help prevent and treat kidney diseases in the future.
Background: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and histopathologic glomerular lesions are among the earliest structural alterations of DN. However, the signaling pathways that initiate these glomerular alterations are incompletely understood.
Methods: To delineate the cellular and molecular basis for DN initiation, we performed single-cell and bulk RNA sequencing of renal cells from type 2 diabetes mice (BTBR ob/ob) at the early stage of DN.