Comparing the performance of the optical glucose assay based on glucose binding protein with high-performance anion-exchange chromatography with pulsed electrochemical detection: efforts to design a low-cost point-of-care glucose sensor.

Background: The glucose binding protein (GBP) is one of many soluble binding proteins found in the periplasmic space of gram-negative bacteria. These proteins are responsible for chemotactic responses and active transport of chemical species across the membrane. Upon ligand binding, binding proteins undergo a large conformational change, which is the basis for converting these proteins into optical biosensors.

Monitoring carbohydrate enzymatic reactions by quantitative in vitro microdialysis.

On-line in vitro microdialysis (MD) sampling followed by HPLC separation and UV absorbance detection (HPLC-UV) was used to monitor carbohydrate enzyme systems. Fundamental parameters (i.e.

Elastic properties of the cell wall of Aspergillus nidulans studied with atomic force microscopy.

Currently, little is known about the mechanical properties of filamentous fungal hyphae. To study this topic, atomic force microscopy (AFM) was used to measure cell wall mechanical properties of the model fungus Aspergillus nidulans. Wild type and a mutant strain (deltacsmA), lacking one of the chitin synthase genes, were grown in shake flasks.

Determination of thio-based additives for biopharmaceuticals by pulsed electrochemical detection following HPLC.

Pulsed electrochemical detection (PED) following liquid chromatographic separation has been applied to the direct (i.e., without derivatization) determination of two major sulfur-containing compounds used as pharmaceutical additives, isopropyl-thio-beta-D-galactopyranoside (IPTG) and monothioglycerol (MTG).