Publications by authors named "Swati Garg"

Introduction: Environmental degradation poses a significant threat, making the comprehension of sustainable behaviour imperative for both environmental and business reasons. Embracing sustainable practices and reducing the unnecessary consumption of resources is essential in our current times. One can be sustainable by focusing on either buying sustainable or green products or by using fewer resources, i.

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One of the fundamental mechanisms developed by the host to contain the highly infectious and rapidly proliferating SARS-coronavirus is elevation of body temperature, a natural fallout of which is heat shock proteins over-expression. Here, for the first time, we demonstrate that the SARS-CoV-2 exploits the host Heat shock protein 70 (Hsp70) chaperone for its entry and propagation, and blocking it can combat the infection. SARS-CoV-2 infection as well as febrile temperature enhanced Hsp70 expression in host Vero E6 cells.

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Emerging Artemisinin (ART) resistance in Plasmodium falciparum (Pf) poses challenges for the discovery of novel drugs to tackle ART-resistant parasites. Concentrated efforts toward the ART resistance mechanism indicated a strong molecular link of ART resistance with upregulated expression of unfolded protein response pathways involving Prefoldins (PFDs). However, a complete characterization of PFDs as molecular players taking part in ART resistance mechanism, and discovery of small molecule inhibitors to block this process have not been identified to date.

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Article Synopsis
  • BRD9 is a protein involved in chromatin remodeling and has potential implications as a target for AML (acute myeloid leukemia), though its role in normal blood cell formation is not fully understood.
  • Inhibition of BRD9 in hematopoietic stem and progenitor cells (HSPCs) results in decreased cell proliferation, loss of stem cells, and impaired differentiation into certain blood cell lineages.
  • RNA sequencing indicates that BRD9 helps maintain active gene transcription during HSPC differentiation, particularly by regulating the master transcription factor GATA1, highlighting its role in the epigenetic regulation of blood cell development.
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Malaria parasite invasion to host erythrocytes is mediated by multiple interactions between merozoite ligands and erythrocyte receptors that contribute toward the development of disease pathology. Here, we report a novel antigen prohibitin "PHB2" and identify its cognate partner "Hsp70A1A" in host erythrocyte that plays a crucial role in mediating host-parasite interaction during merozoite invasion. Using small interfering RNA (siRNA)- and glucosamine-6-phosphate riboswitch (glmS) ribozyme-mediated approach, we show that loss of Hsp70A1A in red blood cells (RBCs) or PHB2 in infected red blood cells (iRBCs), respectively, inhibit PHB2-Hsp70A1A interaction leading to invasion inhibition.

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  • - Palmitoylation, a crucial post-translational modification driven by palmitoyl acyl transferases (PATs), is vital for virulence, necessitating the development of new inhibitors due to issues with existing ones.
  • - Researchers discovered a novel peptide inhibitor, PS1, that specifically targets the PAT6 enzyme, showing effective suppression of palmitoylation in a bacterial model.
  • - PS1 exhibited strong binding to the PAT6-DHHC domain and significantly reduced growth of both promastigotes and amastigotes by lowering cytokine production and invasion capabilities, marking it as a promising candidate for inhibiting pathogen infectivity.
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  • Progress in treating multiple myeloma (MM) has improved survival rates, but there is still a need for better therapies.
  • Research shows that targeting bromodomain-containing protein 9 (BRD9) can block MM cell growth and work effectively with existing treatments called IMiDs by reducing MYC and Ikaros proteins.
  • Combining BRD9 targeting with IMiD treatment not only improves effectiveness against MM but can also overcome resistance in long-term cell cultures, indicating a promising new strategy for treating both MM and resistant cases.
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Introduction: This study empirically investigates the attitude of tobacco and alcohol consumers towards health insurance purchase in India. The study aims to determine the factors which plays a significant role in determining the purchase intention of health insurance among tobacco and alcohol consumers.

Methods: We propose an extended theory of planned behavior (TPB) model comprising factors like attitude, subjective norms, perceived behavior control, perceived usefulness, perceived product risk, and intention to purchase.

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  • Acute myeloid leukemia (AML) is a complex blood cancer that often returns and resists treatment, especially in older patients, prompting the consideration of hematopoietic stem cell transplants as a potential cure.
  • While innovative CAR T-cell therapy has been effective for B-cell cancers, its application in AML has yielded mixed results and highlighted the challenges posed by the disease's genetic diversity.
  • Current efforts are focused on enhancing CAR therapy designs and pinpointing specific AML targets that minimally affect normal blood cells for better treatment outcomes.
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Leishmaniasis is characterized by a spectrum of diseases with two main clinical forms, cutaneous and visceral, caused by and , respectively. Studying 's interaction with the epithelial barrier at the initial site of a bite is crucial to understanding the establishment of the disease. To discern parasite-host epithelial interaction, we developed cellular models involving co-cultures of and MDCK epithelial cells Both -MDCK and -MDCK co-culture models demonstrated a phenomenon known as atypical anoikis apoptosis, typically identified by distinctive 'flipping in' of cell membranes and disordered cytoskeletal frameworks.

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Despite recent advances in the therapy of diffuse large B-cell lymphoma (DLBCL), many patients are still not cured. Therefore, new therapeutic strategies are needed. The anti-apoptotic B-cell lymphoma 2 (BCL2) gene is commonly dysregulated in DLBCL due to various mechanisms such as chromosomal translocation t(14;18)(q32;q21) and copy number alterations; however, targeting BCL-2 with the selective inhibitor, venetoclax, led to response in only a minority of patients.

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Cold shock proteins are characterized by the presence of one or more cold shock domains that bestow them with nucleic acid binding ability. Although cold shock proteins are well characterized in bacteria, plants and humans, there is no information on their existence and role in malaria parasite. Here, we have identified and delineated the function of a cold shock protein of () 'CoSP'.

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Prohibitins (PHBs) are highly conserved pleiotropic proteins as they have been shown to mediate key cellular functions. Here, we characterize PHBs encoding putative genes ofPlasmodium falciparum by exploiting different orthologous models. We demonstrated that PfPHB1 (PF3D7_0829200) and PfPHB2 (PF3D7_1014700) are expressed in asexual and sexual blood stages of the parasite.

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Iron-sulfur (Fe-S) cluster containing proteins have been assigned roles in various essential cellular processes, such as regulation of gene expression, electron transfer, sensing of oxygen and balancing free radical chemistry. However, their role as the drug target remains sparse. Recently the screening of protein alkylation targets for artemisinin in Plasmodium falciparum led to identification of Dre2, a protein involved in redox mechanism for the cytoplasmic Fe-S cluster assembly in different organisms.

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This multi-centre, non-randomized, open-label, phase II trial (NCT03016338), assessed niraparib monotherapy (cohort 1, C1), or niraparib and dostarlimab (cohort 2, C2) in patients with recurrent serous or endometrioid endometrial carcinoma. The primary endpoint was clinical benefit rate (CBR), with ≥5/22 overall considered of interest. Secondary outcomes were safety, objective response rate (ORR), duration of response, progression free survival and overall survival.

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Unlabelled: Drug repurposing is an attractive option for oncology drug development. Itraconazole is an antifungal ergosterol synthesis inhibitor that has pleiotropic actions including cholesterol antagonism, inhibition of Hedgehog and mTOR pathways. We tested a panel of 28 epithelial ovarian cancer (EOC) cell lines with itraconazole to define its spectrum of activity.

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With the proliferation of technological tools and the advancement in electronic devices and accessories, consumers across the world are changing and upgrading their electronic devices at an alarming rate. However, these developments have raised concerns related to electronic waste (E-waste). E-wastes contain toxic substances which may have a negative impact on both humans and the environment.

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The emergence of Plasmodium falciparum resistance raises an urgent need to find new antimalarial drugs. Here, we report the rational repurposing of the anti-hepatitis C virus drug, alisporivir, a nonimmunosuppressive analog of cyclosporin A, against artemisinin-resistant strains of P. falciparum.

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The increased resistance of human malaria parasite Plasmodium falciparum (Pf) to currently used drugs necessities the development of novel anti-malarials. Here, we examine the potential of erythritol, a sugar substitute for therapeutic intervention. Erythritol is a permeant of Plasmodium falciparum aquaglyceroporin (PfAQP) which is a multifunctional channel responsible for maintaining hydro-homeostasis.

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Phosphorylation and other post-translational modifications of red blood cell (RBC) proteins govern membrane function and have a role in the invasion of RBCs by the malaria parasite, . Furthermore, a percentage of RBC proteins are palmitoylated, although the functional consequences are unknown. We establish dynamic palmitoylation of 118 RBC membrane proteins using click chemistry and acyl biotin exchange (ABE)-coupled LC-MS/MS and characterize their involvement in controlling membrane organization and parasite invasion.

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Objective: To evaluate the prognostic role of pretreatment CD4 + , CD8 + T lymphocytes in predicting response to definite chemo radiotherapy in advanced cervical cancer. Design: A hospital-based prospective one-year follow-up study.

Method: This observational study was conducted on 74 patients with advanced cervical cancer.

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Bromodomain-containing protein 9 (BRD9), an essential component of the SWI/SNF chromatin remodeling complex termed ncBAF, has been established as a therapeutic target in a subset of sarcomas and leukemias. Here, we used novel small molecule inhibitors and degraders along with RNA interference to assess the dependency on BRD9 in the context of diverse hematological malignancies, including acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and multiple myeloma (MM) model systems. Following depletion of BRD9 protein, AML cells undergo terminal differentiation, whereas apoptosis was more prominent in ALL and MM.

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