Transfection, cytotoxicity, and cell cycle studies on the two newly developed and characterized humpback grouper (Cromileptes altivelis) fin cell lines.

The development and characterization of two novel humpback grouper (Cromileptes altivelis) fin cell lines are described in this study. The CA1F3Ex and CA1F4Tr cell lines were developed by explant and trypsinization methods, respectively, in Leibovitz's L15 (L-15) medium supplemented with 20% FBS (fetal bovine serum) and subcultured over 150 times. Cell lines exhibited high stability, as evidenced by the high revival rate (85-95%) and good attachment while seeding after one year of cryostorage.

NO Generation from the Cross-Talks between Ene-diol Antioxidants and Nitrite at Metal Sites.

The one-electron reduction of nitrite (NO) to nitric oxide (NO) and ene-diol oxidation are two important biochemical transformations. Employing mononuclear cobalt-nitrite complexes with Co and Co oxidation states, [()Co(nitrite)](ClO) () and [()Co(nitrite)](ClO) (), this report illustrates NO release coupled to stepwise oxidation of ene-diol antioxidants such as l-ascorbic acid (AH) and catechol. Analysis of the AH end-product reveals that the reaction with complex affords dehydroascorbic acid.