Thioredoxin induced antioxidant gene expressions in human lens epithelial cells.

Thioredoxin (Trx) is one of the major redox-regulating proteins. It catalyzes dithiol/disulfide exchange reactions and displays many unique intracellular and extracellular activities thereby controlling multiple mammalian cell functions. In the present study we examine the effect of exogenous Trx on the expression of several antioxidant genes in human lens epithelial (HLE B3) cells.

Human lens thioredoxin: molecular cloning and functional characterization.

Purpose: To molecularly clone the human lens thioredoxin (TXN) gene, characterize the recombinant protein (rTrx1) and study the regulation expression of thioredoxin (Trx1) in human lens epithelial cells under oxidative stress.

Methods: The human TXN gene was cloned from a human lens cDNA library. Trx1 activity was measured by insulin reduction assay.