Publications by authors named "Svetozar Gavrilovic"
Mitochondria play critical roles in regulating cell fate, with dysfunction correlating with the development of multiple diseases, emphasizing the need for engineered nanomedicines that cross biological barriers. Said nanomedicines often target fluctuating mitochondrial properties and/or present inefficient/insufficient cytosolic delivery (resulting in poor overall activity), while many require complex synthetic procedures involving targeting residues (hindering clinical translation). The synthesis/characterization of polypeptide-based cell penetrating diblock copolymers of poly-L-ornithine (PLO) and polyproline (PLP) (PLO-PLP, n:m ratio 1:3) are described as mitochondria-targeting nanocarriers.
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- Nanofabrication has advanced significantly due to DNA origami, which helps create intricate nanoscale structures with potential new biological functions.
- A new method using the E.coli Min protein system allows for the rapid assembly of these DNA structures in about 30 minutes, greatly improving efficiency over older methods.
- This technique enables the creation of various patterns and complex designs, like core-shell patterns, and shows promise for future uses in fields such as plasmonics, catalysis, and molecular sensing.
Article Synopsis
- * The study focuses on how man-made light-activated rotary motors affect biological membranes, revealing significant mechanical changes, including membrane expansion when these motors are activated.
- * Key findings highlight that factors like motor concentration and membrane fluidity impact these changes, with continuous expansion observed as long as the motors are energized, providing insights for future applications in chemical biology.
The bottom-up reconstitution of proteins for their modular engineering into synthetic cellular systems can reveal hidden protein functions in vitro. This is particularly evident for the bacterial Min proteins, a paradigm for self-organizing reaction-diffusion systems that displays an unexpected functionality of potential interest for bioengineering: the directional active transport of any diffusible cargo molecule on membranes. Here, the MinDE protein system is reported as a versatile surface patterning tool for the rational design of synthetically assembled 3D systems.
View Article and Find Full Text PDFNanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure assembly on surfaces using indirect cross-linking through low-complexity connector strands binding staple strand extensions, instead of connector strands binding to scaffold loops.
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