The role of mineral and bone disorders in the development and progression of cardiac and renal pathology in patients with type 1 diabetes mellitus of long duration.

Aims: The objective of our study was to evaluate the role of mineral and bone metabolism disorders associated with chronic kidney disease (MBD-CKD) in the development and progression of cardiac and renal pathology in patients with type 1 diabetes mellitus (T1DM) of long duration.

Methods: We investigated 96 patients with T1DM of long duration, with CKD at different stages (0-5), including patients on hemodialysis (HD) and with kidney transplantation (KT). Along with overall clinical examination, we assessed markers of MBD (calcium, phosphorus, parathormone, vitamin D, fibroblast growth factor (FGF) 23) and levels of cardiac injury marker (atrial natriuretic peptide, NT-proBNP).

Development of a disposable potentiometric sensor for the near patient testing of plasma thiol concentrations.

The reaction of naphthoquinone with physiological thiols has been investigated as the basis of a NPT strategy for the measurement of the latter. Screen-printed carbon electrode assemblies provide an inexpensive and inherently disposable platform for the detection methodology. The key analytical parameters underpinning the selective and sensitive (0.

Potentiometric differentiation of mono- and macromolecular thiol within human plasma at carbon fiber electrodes.

The potentiometric responses of mono- and macromolecular thiol species derivatized with a naphthoquinone indicator have been assessed at electrode designs based on laminated carbon fiber assemblies. The nature of the response for each type of species was found to depend strongly on the electrode geometry and composition and can be used to discriminate between the different thiol moieties. The efficacy of the electrode systems for the measurement of plasma thiols within normal and diabetic blood samples has been assessed, and a possible mechanism for the differing responses has been proposed.