[The status of the gastric and duodenal mucosa, biopsied from those who worked in the cleanup of the aftermath of the accident at the Chernobyl Atomic Electric Power Station, in an organotypic tissue culture].

Biopsies of stomach and duodenum different regions mucosa obtained from patients with gastroduodenitis (liquidators of consequences of accident in Chernobyl nuclear station--LCA CNS-- and control patients) were studied cultured in vitro. The following are the differences found. Mononuclear cells, differentiating into macrophages were exposed from control biopsies.

[The ultrastructural changes in the sympatheic ganglion in experimental hypercholesterolemia].

In conditions of experimental atherisclerosis mature Chinchilla rabbits acquire dystrophic changes of different severeness in neurons, synapses, nervous fibres, perineurium and blood vessel endothelium of the superior cervical sympathetic ga (SCSG) during one month. It is suggested that B-lipoproteins penetrate easily th of SCSG capillaries (of sinusoidal type) and the diffuse neurotissue barrier (pe which leads to structural changes in neuron bodies, axons and synapses. SCSG neurons++ with CNS is disturbed as well as axonal catecholamine transport and neurotrophic of innervated magistral vessels (carotid arteries, aortal arch etc.

[Nerve regeneration under the influence of baliz-2 and laktovit].

Reparative processes in the sciatic nerve of rats were studied by histological methods after a cryogenic injury with a combined and separate action of drugs Lactovit and Balise-2. The animals were divided into 5 groups: I--intact animals; II--control animals; III--animals treated with Balise-2; IV--animals treated with Lactovit; V--animals treated with both Balise-2 and Lactovit. In the nerve trunk within 75 days after injury (the maximum period of observation) the amount of thick myelin fibers with a diameter 6.

Structure and nature of macrophages participating in the wallerian degeneration of nerve fibers.

The posttraumatic processes of Wallerian degeneration of nerves have been illuminated in detail. The dynamics of the breakdown of axons and the myelin sheaths of nerve fibers has been established, as have been the periods of the changes in the composition of myelin, and the reactive changes in the Schwann cells and the connective tissue structures in the makeup of the nerve as well as the formation of "foam" cells have been described. The controversial questions which have been raised in these studies regarding the role of the cellular elements (the Schwann cells, the endoneurial fibroblasts, the cells of the epi- and perineurium) during Wallerian degeneration remain unresolved until the present time.

[Structural-functional changes in the peripheral nerves after administration of several drugs].

Structural and functional changes in the sciatic nerve induced by some drugs were studied using morphological and electrophysiological methods. It was stated that the application of this drug on the nerve causes different degenerating changes. Electrophysiological data, using the regeneration of action potential, proved the presence of structural changes in the nerve after application of mentioned drugs and showed partly preserved conduction of the nerve trunks.

[The structure and nature of the macrophages participating in Wallerian degeneration of nerve fibers].

The nervus ischiadicus in white noninbred rats has been damaged by various means and then light- and electron-microscopically the sources of origin of macrophages, participating in removal of decay products in the distal part of the nerve have been studied. There is a close correlation between the process of Wallerian degeneration and aseptic inflammation. In the area of decay of the myelinated nervous fibers 4 types of cells have been identified and characterized.

[Postinjection damages to the peripheral nerves].

Comparison of pathohistological changes occurring in nerves after injection of agents into them with clinical observations showed that if the toxicity of the agent is sufficiently high to destroy the neuro-tissue barrier, or a less toxic agent is injected under the epineurium then degenerative changes develop in the nerve and their outcome depends on the degree of damage and the tactics of treatment.

[Changes in neural conductors and their end organs in the muscles and skin after major procedures of one-stage lengthening of the extremity by the G.A. Ilizarov method].

The state of the tibial nerve, intramuscular nervous fasciculi and terminals in muscles and skin has been studied in the canine extremities at large one-stage elongations after G. A. Ilizarov.

[Effect of neurite-stimulating protein on the growth and proliferation of peripheral glia cells].

Proliferative activity of peripheral glia was investigated in the organotypic culture of peripheral nerves of 9-10-day old chick embryos. The neurite-stimulating protein, a stimulator of the neurite growth in the organotypic culture of spinal ganglia, being added to the culture media sharply increased the mitotic activity of glia: on the 3d day its level was 3.5 times higher as compared with the control one.

[Changes in the skin epithelium and nerve elements of the rat sole with directed regeneration of the sciatic nerve].

The work has been performed on Wistar rats and non-inbred animals. Their ischiatic nerves have been dissected at the femoral superior third under nembutal narcosis. The end of the sectioned nerve are connected by a fragment of an aorta from rats of the same age.

[Morphofunctional restoration of a nerve trunk by filling the defect with a blood vessel].

Dynamics of morphologic and functional restoration of the nerve trunk and separate receptive skin formations have been investigated in white rats after a sutureless connection of the cut nerve by means of the implanted artery. For testing the reparative processes neurohistological and electrophysiological techniques have been used. In spite of a relatively early restoration of the integrity of the injured nerve and reaching the nervous fibers up to the skin, functional organization of the dermal sensory apparatus is observed only in a year after the operation.

[Development of methods of connecting severed nerve trunks to restore their integrity].

An experimental model of a seamless end-to-end joining of severed sciatic nerve (with gaps between the two ends 5-8 mm-wide) using an implanted arterial vessel was elaborated on random bred and inbred Wistar rats. Histological examination showed that nerve regeneration along the implanted arterial lumen was directly oriented and was not accompanied by neuroma formation which was always the case in the control. It took some nerve fibers 20 days to join the gap between the cut nerve ends.

[Action of epidermal chalone on the vaginal epithelial cell proliferation in ovariectomized mice stimulated by 17 beta-estradiol].

It has been shown that the DNA synthesis inhibitory effect of chalone on the vaginal epithelium of ovariectomized mice administered epidermal chalone three times (8, 4 and 1 h before 17-beta-estradiol injection) is dependent on chalone injection made 1 h before hormone injection. The decrease in the number of DNA synthesizing cells induced by 3-fold injection of chalone during 2 days is linked with the reduction in the level of exogenous estrogen in ovariectomized mice rather than with the duration of epidermal chalone action.

[Effect of epidermal chalone on vaginal epithelial cell proliferation stimulated by estradiol].

Non-inbred and hybrid mice, line C57Bl and CBA in diestrus stage were subjected to ovariectomy and in 4-6 weeks they were given subcutaneously 17-beta-oestradiol (1 or 0.1 mkg per one animal). One hour before the animals were sacrificed, they were given 3H-thymidine intraperitoneally.

[Tissue specific effect of lymphocytic chalone isolated from rat spleen].

Chalone-containing alcohol extract from the rat spleen consisting of 8--10 substances of protein nature, according to the data of the disk electrophoresis, possesses a selective property to inhibit the entrance of human transformed lymphocytes into the phase of DNA synthesis. Injection of lymphocytic chalones to the mice allotransplants of a donor's skin grafts, which differ from the recipient by a strong antigen, temporally delays the rejection of the transplanted graft. In mice-recipients, the thymus treated with lymphocytic chalone is characterized by the depletion of the cortical substance.

[Proliferative processes in the epithelium of the autotransplant and surrounding skin of mice].

Proliferative processes were studied in epithelial cells of skin autografts and the surrounding skin in mice of CBA line. In the animals subjected to the operation but without cortisone injection (Fig. 1, a and Fig.

[Proliferative processes in the epithelium of mouse skin allotransplants following cortisone administration].

Proliferative processes were studied in the epithelium of skin grafts from mice of strain CC57 Brown and that of the skin surrounding the grafts in mice of CBA strain. The chart (Fig 1) illustrates cortisone action on normalization of DNA synthesis in the epithelial cells of the allotransplant. Throughout the experiment in operated animals which received no cortisone (Fig.

[Influence of cortisone on the implantation of skin allotransplants].

Skin transplants of CC57 Brown mice to CBA mice different in strong antigens controlled by H-2 locus of histocompatibility were studied with special attention given to the division of epithelial cells. Five-day-long injecting of cortisone (single dose 2,5 mg) was performed in 2 variants of experiments: 2 days before or 3 days after operation. The mean day of rejection of the graft after injection of the hormone was prolonged from 6,9 to 17,8--19,8 days.