Physiological basis for atmospheric methane oxidation and methanotrophic growth on air.

Atmospheric methane oxidizing bacteria (atmMOB) constitute the sole biological sink for atmospheric methane. Still, the physiological basis allowing atmMOB to grow on air is not well understood. Here we assess the ability and strategies of seven methanotrophic species to grow with air as sole energy, carbon, and nitrogen source.

Thermal acclimation of methanotrophs from the genus Methylobacter.

Methanotrophs oxidize most of the methane (CH) produced in natural and anthropogenic ecosystems. Often living close to soil surfaces, these microorganisms must frequently adjust to temperature change. While many environmental studies have addressed temperature effects on CH oxidation and methanotrophic communities, there is little knowledge about the physiological adjustments that underlie these effects.

Draft genome sequence data of a psychrophilic tundra soil methanotroph, Z-0021 (DSM 9914).

Psychrophilic methanotrophic bacteria are abundant and play an important role in methane removal in cold methanogenic environments, such as boreal and arctic terrestrial and aquatic ecosystems. They could be also applied in the bioconversion of biogas and natural gas into value-added products (e.g.

A combined microbial and biogeochemical dataset from high-latitude ecosystems with respect to methane cycle.

High latitudes are experiencing intense ecosystem changes with climate warming. The underlying methane (CH) cycling dynamics remain unresolved, despite its crucial climatic feedback. Atmospheric CH emissions are heterogeneous, resulting from local geochemical drivers, global climatic factors, and microbial production/consumption balance.

Characterization and genome analysis of a psychrophilic methanotroph representing a ubiquitous Methylobacter spp. cluster in boreal lake ecosystems.

Lakes and ponds are considered as a major natural source of CH emissions, particularly during the ice-free period in boreal ecosystems. Aerobic methane-oxidizing bacteria (MOB), which utilize CH using oxygen as an electron acceptor, are one of the dominant microorganisms in the CH-rich water columns. Metagenome-assembled genomes (MAGs) have revealed the genetic potential of MOB from boreal aquatic ecosystems for various microaerobic/anaerobic metabolic functions.

Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane.

Methane (CH) is a sustainable carbon feedstock for value-added chemical production in aerobic CH-oxidizing bacteria (methanotrophs). Under substrate-limited (e.g.

Correction: Rainer et al. The Influence of Above-Ground Herbivory on the Response of Arctic Soil Methanotrophs to Increasing CH Concentrations and Temperatures. 2021, , 2080.

The authors wish to make the following corrections to this paper [...

The Influence of Above-Ground Herbivory on the Response of Arctic Soil Methanotrophs to Increasing CH Concentrations and Temperatures.

Rising temperatures in the Arctic affect soil microorganisms, herbivores, and peatland vegetation, thus directly and indirectly influencing microbial CH production. It is not currently known how methanotrophs in Arctic peat respond to combined changes in temperature, CH concentration, and vegetation. We studied methanotroph responses to temperature and CH concentration in peat exposed to herbivory and protected by exclosures.

Draft genome sequence data of methanotrophic strain S1L and strain S2AM isolated from hypoxic water column layers of boreal lakes.

Methanotrophic bacteria inhabit a wide range of natural (e.g. wetlands, lakes and soils) and anthropogenic (e.

Decoupling of microbial community dynamics and functions in Arctic peat soil exposed to short term warming.

Temperature is an important factor governing microbe-mediated carbon feedback from permafrost soils. The link between taxonomic and functional microbial responses to temperature change remains elusive due to the lack of studies assessing both aspects of microbial ecology. Our previous study reported microbial metabolic and trophic shifts in response to short-term temperature increases in Arctic peat soil, and linked these shifts to higher CH and CO production rates (Proceedings of the National Academy of Sciences of the United States of America, 112, E2507-E2516).

Life-history genomic regions explain differences in Atlantic salmon marine diet specialization.

Animals employ various foraging strategies along their ontogeny to acquire energy, and with varying degree of efficiencies, to support growth, maturation and subsequent reproduction events. Individuals that can efficiently acquire energy early are more likely to mature at an earlier age, as a result of faster energy gain which can fuel maturation and reproduction. We aimed to test the hypothesis that heritable resource acquisition variation that covaries with efficiency along the ontogeny would influence maturation timing of individuals.

Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth.

The second largest sink for atmospheric methane (CH) is atmospheric methane oxidizing-bacteria (atmMOB). How atmMOB are able to sustain life on the low CH concentrations in air is unknown. Here, we show that during growth, with air as its only source for energy and carbon, the recently isolated atmospheric methane-oxidizer MG08 (USCα) oxidizes three atmospheric energy sources: CH, carbon monoxide (CO), and hydrogen (H) to support growth.

Environmental patterns of brown moss- and Sphagnum-associated microbial communities.

Northern peatlands typically develop through succession from fens dominated by the moss family Amblystegiaceae to bogs dominated by the moss genus Sphagnum. How the different plants and abiotic environmental conditions provided in Amblystegiaceae and Sphagnum peat shape the respective moss associated microbial communities is unknown. Through a large-scale molecular and biogeochemical study spanning Arctic, sub-Arctic and temperate regions we assessed how the endo- and epiphytic microbial communities of natural northern peatland mosses relate to peatland type (Sphagnum and Amblystegiaceae), location, moss taxa and abiotic environmental variables.

The Impact of Methane on Microbial Communities at Marine Arctic Gas Hydrate Bearing Sediment.

Cold seeps are characterized by high biomass, which is supported by the microbial oxidation of the available methane by capable microorganisms. The carbon is subsequently transferred to higher trophic levels. South of Svalbard, five geological mounds shaped by the formation of methane gas hydrates, have been recently located.

Renal cell carcinoma presenting as a tumor on the scalp: A case report.

Introduction: Renal cell carcinoma (RCC) is often diagnosed as an incidental finding on imaging studies and about a fourth of patients have metastases by the time of diagnosis. RCC is known to metastasize widely but cutaneous metastases are considered uncommon and are rarely the presenting symptom of RCC. We present a case of RCC presenting with a tumor on the scalp.

[Atypical presentation of lentigo maligna melanoma].

Lentigo maligna melanoma (LMM) is the most common subtype of melanoma in the face. In this case report, a 95-year-old woman had a patch of dark hair growing out of her white hair on her scalp. A punch biopsi confirmed the diagnosis of LMM.

Methanotroph populations and CH4 oxidation potentials in high-Arctic peat are altered by herbivory induced vegetation change.

Methane oxidizing bacteria (methanotrophs) within the genus Methylobacter constitute the biological filter for methane (CH4) in many Arctic soils. Multiple Methylobacter strains have been identified in these environments but we seldom know the ecological significance of the different strains. High-Arctic peatlands in Svalbard are heavily influenced by herbivory, leading to reduced vascular plant and root biomass.

Anaerobic oxidation of methane and associated microbiome in anoxic water of Northwestern Siberian lakes.

Arctic lakes emit methane (CH) to the atmosphere. The magnitude of this flux could increase with permafrost thaw but might also be mitigated by microbial CH oxidation. Methane oxidation in oxic water has been extensively studied, while the contribution of anaerobic oxidation of methane (AOM) to CH mitigation is not fully understood.

Frenulate siboglinids at high Arctic methane seeps and insight into high latitude frenulate distribution.

Frenulate species were identified from a high Arctic methane seep area on Vestnesa Ridge, western Svalbard margin (79°N, Fram Strait) based on mitochondrial cytochrome oxidase subunit I (mtCOI). Two species were found: , and a new, distinct, and undescribed species. The new species adds to the cryptic species complex found at high latitude methane seeps in the north Atlantic and the Arctic.

Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments.

Sedimentary biofilms comprising microbial communities mediating the anaerobic oxidation of methane are rare. Here, we describe two biofilm communities discovered in sediment cores recovered from Arctic cold seep sites (gas hydrate pingos) in the north-western Barents Sea, characterized by steady methane fluxes. We found macroscopically visible biofilms in pockets in the sediment matrix at the depth of the sulphate-methane-transition zone.

Widespread soil bacterium that oxidizes atmospheric methane.

The global atmospheric level of methane (CH), the second most important greenhouse gas, is currently increasing by ∼10 million tons per year. Microbial oxidation in unsaturated soils is the only known biological process that removes CH from the atmosphere, but so far, bacteria that can grow on atmospheric CH have eluded all cultivation efforts. In this study, we have isolated a pure culture of a bacterium, strain MG08 that grows on air at atmospheric concentrations of CH [1.

[A wooden splint under a fingernail led to subungual squamous cell carcinoma].

This case report is about a 75-year-old man, who developed a subungual squamous cell carcinoma (SCC) after having a wooden splint sitting under his fingernail for 20 years. There were no signs of infection, inflammation or tumour. The splint was removed, and histology indicated SCC.

Cryptic frenulates are the dominant chemosymbiotrophic fauna at Arctic and high latitude Atlantic cold seeps.

We provide the first detailed identification of Barents Sea cold seep frenulate hosts and their symbionts. Mitochondrial COI sequence analysis, in combination with detailed morphological investigations through both light and electron microscopy was used for identifying frenulate hosts, and comparing them to Oligobrachia haakonmosbiensis and Oligobrachia webbi, two morphologically similar species known from the Norwegian Sea. Specimens from sites previously assumed to host O.

Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples.

Here we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplified16S rRNA genes recovered from biomass naturally present in the soils by one log unit.