Glutathione has cell protective and anti-catabolic effects in articular cartilage without impairing the chondroanabolic phenotype.

Joint injuries and consequent oxidative stress is a high-risk factor for developing post-traumatic osteoarthritis (OA). While antioxidative therapy using N-acetylcysteine (NAC) has cell- and chondroprotective effects following cartilage injury, it strongly impairs matrix synthesis. Consequently, direct application of Glutathione (GSH) was tested as an alternative therapeutic approach using an cartilage trauma model and isolated chondrocytes, with comparison to NAC.

Senolytic therapy combining Dasatinib and Quercetin restores the chondrogenic phenotype of human osteoarthritic chondrocytes by the release of pro-anabolic mediators.

Cellular senescence is associated with various age-related disorders and is assumed to play a major role in the pathogenesis of osteoarthritis (OA). Based on this, we tested a senolytic combination therapy using Dasatinib (D) and Quercetin (Q) on aged isolated human articular chondrocytes (hACs), as well as in OA-affected cartilage tissue (OARSI grade 1-2). Stimulation with D + Q selectively eliminated senescent cells in both, cartilage explants and isolated hAC.

Simvastatin and fluvastatin attenuate trauma-induced cell death and catabolism in human cartilage.

Joint injuries are known to induce pathomechanisms that might lead to posttraumatic osteoarthritis (PTOA). In this regard, statins with their pleiotropic effects could represent potential therapeutic agents in preventing the development of PTOA. Therefore, we investigated the effects of simvastatin and fluvastatin in a drop-tower-based human cartilage trauma model.

Quantification of 3R,3'R-zeaxanthin in plant derived food by a diastereomeric dilution assay applying chiral high-performance liquid chromatography.

For quantification of 3R,3'R-zeaxanthin in plant derived food, a new diastereomeric dilution assay (DIDA) was developed using chiral high-performance liquid chromatography coupled to diode-array detection (HPLC/DAD). As diastereomeric internal standard (I.S.