Publications by authors named "Svenja Mareike Pauer"
Background: Intestinal organoids are stem cell-derived, 3D "mini-guts" with similar functions as the native intestinal epithelium such as electrolyte transport or establishment of an epithelial barrier. During intestinal inflammation, epithelial functions are dysregulated by proinflammatory cytokines like tumor necrosis factor α (TNFα) and other messengers from the immune system resulting in a loss of electrolytes and water due to an impaired epithelial barrier and higher net secretion.
Methods: A murine small intestinal organoid model was established to study (long-term) effects of TNFα on the intestinal epithelium using live imaging, immunohistochemical staining and qPCR.