Publications by authors named "Svenja Bolz"
Article Synopsis
- Communication in the nervous system involves calcium-triggered neurotransmitter release through exocytosis of synaptic vesicles (SVs) at specific sites.
- Although significant progress has been made in understanding the molecular details of SV fusion, the processes for SV formation during synapse development and their reformation after exocytosis remain less understood.
- The review highlights current knowledge about SV precursor organelles' formation and transport, critiques the mechanisms of SV reformation in mature neurons, and identifies areas for future research exploration.
The minor phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P] is crucial for neurotransmission and has been implicated in Parkinson's disease. Here, we present a staining protocol for the analysis of activity-dependent changes of PI(4,5)P at synapses. We describe steps for stimulating and fixing murine hippocampal neurons, staining with probes for PI(4,5)P and a synaptic marker, and analysis by high-resolution microscopy.
View Article and Find Full Text PDFExocytosis and endocytosis are essential physiological processes and are of prime importance for brain function. Neurotransmission depends on the Ca-triggered exocytosis of synaptic vesicles (SVs). In neurons, exocytosis is spatiotemporally coupled to the retrieval of an equal amount of membrane and SV proteins by compensatory endocytosis.
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