Enhancing endometrial cancer detection: Blood serum intrinsic fluorescence data processing and machine learning application.

Endometrial cancer (EC) is the most prevalent cancer within the female reproductive system in developed countries. Despite its high incidence, there is currently no established laboratory screening test for EC, making early detection challenging. This study introduces an innovative, minimally invasive, and cost-effective method utilizing three-dimensional fluorescence analysis combined with machine learning algorithms to enhance early EC detection.

Non-Invasive Endometrial Cancer Screening through Urinary Fluorescent Metabolome Profile Monitoring and Machine Learning Algorithms.

Endometrial cancer is becoming increasingly common, highlighting the need for improved diagnostic methods that are both effective and non-invasive. This study investigates the use of urinary fluorescence spectroscopy as a potential diagnostic tool for endometrial cancer. Urine samples were collected from endometrial cancer patients ( = 77), patients with benign uterine tumors ( = 23), and control gynecological patients attending regular checkups or follow-ups ( = 96).

Illusory movements for immobile patients with extensive burns (IMMOBILE): A randomized, controlled, cross-over trial.

Introduction: Patients who have sustained extensive burns frequently exhibit substantial damage to skeletal muscle and associated complications. The rehabilitation of these patients can be challenging due to the nature of the injury and the subsequent complications. Nevertheless, there is a possibility that functional proprioceptive stimulation (illusory movements) may facilitate effective rehabilitation in patients with limited physiotherapy options.

Potential application of body fluids autofluorescence in the non-invasive diagnosis of endometrial cancer.

Background: Endometrial carcinoma (EC) is the most common cancer of the female reproductive tract in developed countries. The prognosis and 5-year survival rates are closely tied to the stage diagnosis. Current routine diagnostic methods of EC are either lacking specificity or are uncomfortable, invasive and painful for the patient.

Unveiling the Crucial Role of Chemical Enhancement in the SERS Analysis of Amphetamine-Metal Interactions on Gold and Silver Surfaces: Importance of Selective Amplification of the Narrow Interval of Vibrational Modes.

The use of addictive substances, including drugs, poses significant health risks and contributes to various social problems, such as increased crime rates associated with substance-induced aggressive behavior. To address these challenges, possession of addictive substances is legally prohibited. However, detecting and analyzing these substances remain a complex task for law enforcement, primarily due to the presence of adulterants or limited sample quantities.

Laser-Induced Reactions of 4-Aminobenzenthiol Species Adsorbed on Ag, Au, and Cu Plasmonic Structures Followed by SERS Spectroscopy. The Role of Substrate and Excitation Energy - Surface-Complex Photochemistry and Plasmonic Catalysis.

This study focuses on investigating the laser-induced reactions of various surface complexes of 4-aminobenzenethiol on Ag, Au, and Cu surfaces. By utilizing different excitation wavelengths, the distinct behavior of the molecule species on the plasmonic substrates was observed. Density functional theory (DFT) calculations were employed to establish the significant role of chemical enhancement mechanisms in determining the observed behavior.

Nano-FTIR spectroscopy of surface confluent polydopamine films - What is the role of deposition time and substrate material?

Polydopamine (PDA) is a widely used anchoring layer for multiple purposes. While simple to prepare, PDA is characterized by high chemical and topological diversity, which can limit its versatility. Unraveling the formation mechanism and physicochemical properties of continuous confluent layer and adherent nanoparticles on the nanoscale is crucial to further extend the prospective applications of PDA.

Adult education needs inventory: Construction and application.

Background: The article presents the psychometric parameters and implementation of the adult education needs inventory (AENI) questionnaire, which is designed to measure the key competences of adult educators. It was constructed on the concept of key competences as proposed by the Council of the European Union. This model of competences is inscribed in the concept of lifelong learning and does not concentrate on the compensatory functions of the competences but merely promotes the insight of educators into their self-development.

Chiral, Magnetic, and Photosensitive Liquid Crystalline Nanocomposites Based on Multifunctional Nanoparticles and Achiral Liquid Crystals.

Nanoparticles serving as a multifunctional and multiaddressable dopant to modify the properties of liquid crystalline matrices are developed by combining cobalt ferrite nanocrystals with organic ligands featuring a robust photosensitive unit and a source of chirality from the natural pool. These nanoparticles provide a stable nanocomposite when dispersed in achiral liquid crystals, giving rise to chiral supramolecular structures that can respond to UV-light illumination, and, at the same time, the formed nanocomposite possesses strong magnetic response. We report on a nanocomposite that shows three additional functionalities (chirality and responsiveness to UV light and magnetic field) upon the introduction of a single dopant into achiral liquid crystals.

Killing Effect of FZB42 on a pv. (Xcc) Strain Newly Isolated from Cabbage Convar. (L.): A Metabolomic Study.

The potential use of FZB42 for biological control of various phytopathogens has been documented over the past few years, but its antagonistic interactions with xanthomonads has not been studied in detail. Novel aspects in this study consist of close observation of the death of pv. cells in a co-culture with FZB42, and quantification of lipopeptides and a siderophore, bacillibactin, involved in the killing process.

Vibrational spectroscopic study of selected alkaloids with therapeutic effects.

Nowadays, scientists from various fields of chemistry, biochemistry or biology are interested in trace detection of different natural or synthetic substances such as alkaloids which can either positively or negatively affect human physical and mental health. Linked with that, growing interest in broad applications of advanced vibrational spectroscopic techniques encourage higher demands in this rapidly developing field. This study is focused on a detailed description of infrared and Raman spectra of two natural alkaloids (namely galantamine and buprenorphine) and study of their optical response in the vicinity of gold and silver nanostructured surface.

NK cells not only in endometrium but also in ovulatory cervical mucus in patients with decreased fertility.

Objective: An increased number of NK cells is associated with autoimmune disorder and is known to play a role in infertility. The aim of our research was to monitor the density of NK cells CD56+ and CD16+ in ovulatory cervical mucus (OCM) and in endometrium in infertile women as well as in connection with the actual status of antibodies against phospholipids, sperm and HHV-6 antibodies.

Type Of Study: Original aticle.

Detection and identification of medically important alkaloids using the surface-enhanced Raman scattering spectroscopy.

Currently, trace detection of drugs, medicinal products, psychoactive substances, poisons and other natural or synthetic compounds in the human body has become one of the most important areas of interest in medicine, toxicology and forensic research. Due to the rapid development of nanotechnology, applications in forensic and biological sciences, food industry and art preservation there is an increasing interest in surface-enhanced Raman scattering (SERS) spectroscopy as a technique capable of low detection limits in the analysis of small amounts of studied analytes. In this study, different excitation wavelengths (785 nm and 1064 nm) were used to find the appropriate experimental conditions for the detection and identification of medically significant alkaloids - atropine and pergolide - by means of surface-enhanced Raman scattering spectroscopy.

Preparation of surfaces of composite samples for tip based micro-analyses using ion beam milling.

Ion beam milling, as a method of surface design for tip analytical techniques, was explored. A sample of clay, embedded in a resin, was treated by the ion beam and allowed AFM (a typical tip technique) to be successfully applied. The method is suitable for advanced tip analyses based on AFM, like TERS or SNOM, and for samples that are not possible to prepare by standard mechanical methods.

The use of infrared spectroscopic techniques to characterize nanomaterials and nanostructures: A review.

Recent advances in nanotechnology have opened a lot of new possibilities for nanomaterials application in wide variety of industrial, pharmaceutical, medicinal and environmental applications. This review aims to description of various Fourier Transform Infrared (FTIR)-based spectroscopic techniques suitable to characterize (i) different types of nanomaterials and (ii) various macroscopic samples at their nanoscale. In the introductory section, nanomaterials are classified according to their crucial properties, i.

Synthesis and Properties of Nanosized Stoichiometric Cobalt Ferrite Spinel.

Nanoparticles with controllable sizes of ferrite spinel CoFe₂O₄ were formed by thermal treatment of cobalt-iron glycerolate. Thermal behavior during the heating was studied by differential thermal analysis combined with thermogravimetry. The precursor, as well as the prepared nanoparticles, were analyzed by a broad spectrum of analytic techniques (X-Ray photoelectron spectroscopy (XPS), X-Ray diffraction (XRD), Energy dispersive spectroscopy (EDS), Atomic absorption spectroscopy (AAS), Scanning electron microscopy (SEM), and Raman spectroscopy).

SERS study of riboflavin on green-synthesized silver nanoparticles prepared by reduction using different flavonoids: What is the role of flavonoid used?

Spectroscopy of surface-enhanced Raman scattering (SERS) is nowadays widely used in the field of bio-science and medicine. These applications require new enhancing substrates with special properties. They should be non-toxic, environmentally friendly and (bio-) compatible with examined samples.

[Mumps outbreak in the Plzeň Region in 2011].

Aim: The mumps outbreak in the Plzeň Region in 2011 was analysed retrospectively using the epidemiological, clinical, and laboratory data. Vaccine efficacy analysis was also conducted in various population groups.

Material And Methods: The routine procedure and standard form were used by the epidemiologists to collect data on the age, sex, place of residence, presence in collectivities, date of disease onset, type of complications and date of their onset, hospital admission, vaccination, and results of laboratory analyses.

[Cytological-energetic principle of the amniotic fluid examination on female patients with diagnosis of preterm premature rupture of membranes - up to now experiences and perspectives].

In our study we have dealt with the amniotic fluid examination on female patients with diagnosis of preterm premature rupture of membranes (PPROM). For one of the most important task in the feto-maternal medicine we consider the discovered method of amniotic fluid examination which is capable of the earliest possible detection in an intraamniotic inflammatory response. Unnoticed could harm or in the most serious cases threat the life of fetus or its mother.

[Enterovirus sequencing as a new approach to the laboratory diagnosis for clinical and epidemiological purposes].

Objective: Introducing enterovirus sequencing as an advanced approach to classify the viruses isolated according to the novel nomenclature and to characterize isolates in detail.

Material And Methods: Seventy-five specimens collected from 64 patients in two hospitals, Liberec Regional Hospital, and Plzeň University Hospital, were analyzed. The study patients' age ranged from four to 54 years, with a median of 15 years in males and 16 years in females.

Through-the-wall localization of a moving target by two independent ultra wideband (UWB) radar systems.

In the case of through-the-wall localization of moving targets by ultra wideband (UWB) radars, there are applications in which handheld sensors equipped only with one transmitting and two receiving antennas are applied. Sometimes, the radar using such a small antenna array is not able to localize the target with the required accuracy. With a view to improve through-the-wall target localization, cooperative positioning based on a fusion of data retrieved from two independent radar systems can be used.

Tick-borne encephalitis in children and adolescents in the Czech Republic between 1960 and 2007.

Background: The Czech Republic ranks among the countries with the highest prevalence of tick-borne encephalitis worldwide. The region of West Bohemia has the second highest morbidity within the Czech Republic.

Methods: Between 1960 and 2007, laboratories confirmed 410 cases of tick-borne encephalitis in children and adolescents of West Bohemia.

The diagnosis of norovirus infections in hospitalized children and adolescents with acute gastroenteritis: a study from Pilsen, Czech Republic.

Background: Norovirus-associated sporadic gastroenteritis is an important cause of illness in Western Europe. However, at present, little information on the role of norovirus in sporadic gastroenteritis in Central Europe is available. Our study aimed at providing an assessment of their significance in hospitalized children and adolescents with acute gastroenteritis using ELISA test at the time of their introduction.