Effect of separate and combined exposure of selenium and diazinon on rat sperm motility by computer assisted semen analysis.

Effects of selenium (Se) and diazinon (DZN) on sperm motility parameters in rats were investigated. Male rats received a separate dose of Se (2mgkg b.w.

Analysis of the expression of platelet antigens CD9 and CD41/61 in transgenic rabbits with the integrated human blood clotting factor VIII gene construct.

The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously compared with the expression of the same molecules of non-transgenic rabbits. The immunochemical analysis by indirect immunofluorescence, ELISA and indirect immunoperoxidase staining of blood cells and udder tissues show that the insertion of the WAP-hFVIII gene construct into the rabbit genome, do not influence the expression of cell membrane antigens CD9 and CD41/61 on the blood platelets, polymorphonuclear blood cells, milk somatic cells and mammary gland tissues.

Biochemical and histochemical characterization of the cattle V red blood cell antigen with monoclonal antibody IVA-41.

The cattle V antigen from the FV blood group system was characterized. Hemolytic as well as immunochemical analyses with monoclonal antibody (MAb) IVA-41 found that V antigen of bovine red blood cells is a membrane-bound, papain- and pronase-sensitive, trypsin- and chymotrypsin-resistant N-glycosylated sialoglyco-protein with molecular weight of 64, 56, and 50 kDa under no reduction and 23 kDa under reduction conditions. In contrary to some human blood group antigens, the expression of bovine blood group V antigen is restricted to the erythrocyte membrane.

Monoclonal antibody to the light chain of bovine immunoglobulin.

Monoclonal antibody IVA-285 (IgG1 isotype) recognizing antigenic determinant on bovine and ovine immunoglobulin light chain was produced and characterized. Western blot analysis of bovine immunoglobulin G (IgG) and immunoglobulin M (IgM) purified from bovine blood serum as well as whole immunoglobulin fractions of bovine and ovine serum with IVA-285 showed a molecular weight in the 24-27 kd range corresponding to the Ig light chain of bovine Ig. IVA-285 recognizes the Ig light chain on Ig+ lymphocyte subpopulation and in the majority of body fluids; however, especially strong reactions were observed in bovine tissues (lymph node follicles, plasma cells, and Ig deposits in various tissues).

The influence of ultra-violet radiation on chicken hatching.

The influence of UV-B radiation on embryonic development of chickens Hampshire breed was investigated. The set eggs with the average weight of 60.0 +/- 0.