Assessing the performance of quantity and quality metrics using the QIAGEN Investigator® Quantiplex® pro RGQ kit.

The Quantiplex® Pro RGQ kit quantifies DNA in a sample, supports the detection of mixtures and assesses the extent of DNA degradation based on relative ratios of amplified autosomal and male markers. Data show no significant difference in the accuracy and sensitivity of quantification between this and the Promega PowerQuant® System, both detecting the lowest amount of DNA tested, 4 pg. Laboratory controlled mixed male:female DNA samples together with mock sexual assault samples were quantified across a range of mixture ratios.

An RNA expression method for aging forensic hair samples.

A common limitation to most forensic trace evidence analysis is the ability to determine the time at which the evidence was deposited at the crime scene. This issue of timing is vitally important as it may not only reveal when the crime occurred, but could exclude potential suspects from the investigation. Using a reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, we monitored the relative expression ratio (RER) of two different RNA species (18S and β-actin) in hair samples that were aged naturally over a period of 3 months.