Protective effect of antioxidant peptides from grass carp scale gelatin on the HO-mediated oxidative injured HepG2 cells.

The protective effect of antioxidant peptides from grass carp scale gelatin on hydrogen peroxide (HO)-mediated oxidative injured HepG2 cells was investigated, and the protective mechanism as well as peptide structure were studied. Pretreated with grass carp scale gelatin hydrolysates (GSGH) for 24 h significantly increased the survival rates and decreased the apoptosis rates of HO-mediated oxidative injured HepG2 cells. The increase in SOD, CAT and GPX activities, reduce in ROS level and MDA content, and weaken in damage on cell membrane and DNA could be responsible for the protective effect of GSGH on HO-mediated oxidative injured HepG2 cells.

Understanding reproducibility of human IVF traits to predict next IVF cycle outcome.

Purpose: Evaluating the failed IVF cycle often provides useful prognostic information. Before undergoing another attempt, patients experiencing an unsuccessful IVF cycle frequently request information about the probability of future success. Here, we introduced the concept of reproducibility and formulae to predict the next IVF cycle outcome.

Optimal use of fresh and frozen-thawed testicular sperm for intracytoplasmic sperm injection in azoospermic patients.

Purpose: To optimize the use of fresh and frozen-thawed testicular biopsy specimens from patients with azoospermia.

Methods: Fifty-one patients suffering from obstructive and non-obstructive azoospermia underwent testicular sperm extraction (TESE). The specimens were divided and used for either in vitro maturation or freezing for a future intracytoplasmic sperm injection (ICSI) cycle.