Cell type-specific filamin complex regulation by a novel class of HECT ubiquitin ligase is required for normal cell motility and patterning.

Differential cell motility, which plays a key role in many developmental processes, is perhaps most evident in examples of pattern formation in which the different cell types arise intermingled before sorting out into discrete tissues. This is thought to require heterogeneities in responsiveness to differentiation-inducing signals that result in the activation of cell type-specific genes and 'salt and pepper' patterning. How differential gene expression results in cell sorting is poorly defined.

Rapid high-performance liquid chromatographic analysis of adenovirus type 5 particles with a prototype anion-exchange analytical monolith column.

To support effective process development there is a requirement for rapid analytical methods that can identify and quantitate adenoviral particles throughout the manufacturing process, from cellular lysate through to purified adenovirus. An anion-exchange high-performance liquid chromatography method for the analysis of adenovirus type 5 (Ad5) particles has been developed using a novel quaternary amine monolithic column (Bio-Monolith QA, Agilent). The developed method separates intact Ad5 from contaminating proteins and DNA, thus allowing analysis of non-purified samples during process development.