Chinese Giant Salamander Iridovirus 025L Is a Viral Essential Gene.

Ranavirus is a large nucleocytoplasmic DNA virus. Chinese giant salamander iridovirus (CGSIV) belongs to the ranavirus genus, and its replication involves a series of essential viral genes. Viral is a gene closely associated with viral replication.

Detection of single influenza viral RNA in cells using a polymeric sequence probe.

A polynucleotide probe, call a polymeric sequence probe (PSP), was used to detect influenza A (Influenza A/WSN/33) NA (Neuraminidase) viral RNA in Madin-Darby canine kidney (MDCK) cells. The PSP is a single-stranded DNA molecule with ~2,000 tandem repeat fluorescence binding sites and target binding sites that can bind with multiple fluorescence complementary oligos and target viral RNA using a fluorescence in situ hybridization (FISH) process. A single viral RNA labeled by PSP can be directly observed in MDCK cells.

Polymeric sequence probe for single DNA detection.

A polynucleotide probe, polymeric sequence probe (PSP), was developed for single molecular detections. PSP is a single-stranded DNA molecule with ~2000 tandem repeat target-binding sequences and label-binding sequences. A single PSP can bind to multiple fluorescent complementary oligos to generate a strong fluorescence signal.

Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds.

The effective and sustained delivery of DNA and siRNAs locally would increase the applicability of gene therapy in tissue regeneration and cancer therapy. One promising approach is to use hydrogel scaffolds to encapsulate and deliver nucleotides in the form of nanoparticles to the disease sites. However, this approach is currently limited by the inability to load concentrated and active gene delivery nanoparticles into the hydrogels due to the severe nanoparticle aggregation during the loading process.

Ultrasensitive fluorescence detection of single protein molecules manipulated electrically on Au nanowire.

Proteins assembled on an Au nanowire are manipulated by an electrical potential applied on the nanowire, which leads to the modulation of molecular fluorescence. The molecular modality can be unequivocally correlated with the modulated fluorescence, which enables the specific fluorescence from a single target protein to be unambiguously distinguished from background noise and nonspecific fluorescence. As demonstrated through a thrombin assay, this simple method can significantly improve the sensitivity and specificity of the protein detection down to the single molecule level.

Dynamically configurable biomolecular nanoarrays.

Nanoarrays of distinct DNA and protein biomolecules were fabricated by electrochemically controlling their assembly/release from Au nanoelectrodes on a chip. The surface density, ratio, and activity of the biomolecules assembled on each nanoelectrode in the array can be configured quantitatively and temporally by adjusting the electrochemical potential applied on the nanoelectrode. The dynamically configurable biomolecular nanoarray can potentially activate combinatorial interactions with microbiosystems under the control of an electronic circuit for biological and medical applications.