Mitochondrial Genome Insights into Evolution and Gene Regulation in .

As a globally distributed perennial Gramineae, can adapt to harsh ecological environments and has significant economic and environmental values. Here, we performed a complete assembly and annotation of the mitogenome of using genomic data from the PacBio and BGI platforms. The mitogenome is a multibranched structure of 501,134 bp, divided into two circular chromosomes of 325,493 bp and 175,641 bp, respectively.

Telomere-to-telomere Phragmites australis reference genome assembly with a B chromosome provides insights into its evolution and polysaccharide biosynthesis.

Phragmites australis is a globally distributed grass species (Poaceae) recognized for its vast biomass and exceptional environmental adaptability, making it an ideal model for studying wetland ecosystems and plant stress resilience. However, genomic resources for this species have been limited. In this study, we assembled a chromosome-level reference genome of P.

Transcriptome Analysis Reveals the Crucial Role of Phenylalanine Ammonia-Lyase in Low Temperature Response in .

: is a rare temperate evergreen shrub with high tolerance to low temperature, and understanding the related gene expression regulatory network can help advance research on the mechanisms of plant tolerance to abiotic stress. Here, time-course transcriptome analysis was applied to investigate the gene expression network in under low temperature stress. : A total of 12,606 differentially expressed genes (DEGs) were identified at four time-points during low temperature stress treatment, and multiple pathways, such as plant hormones, secondary metabolism, and cell membranes, were significantly enriched in the DEGs.

Historical Blurry Video-Based Face Recognition.

Face recognition is a widely used computer vision, which plays an increasingly important role in user authentication systems, security systems, and consumer electronics. The models for most current applications are based on high-definition digital cameras. In this paper, we focus on digital images derived from historical motion picture films.

The dehydration-responsive protein PpFAS1.3 in moss Physcomitrium patens plays a regulatory role in lipid metabolism.

Moss plants appear in the early stages of land colonization and possess varying degrees of dehydration tolerance. In this study, a protein called PpFAS1.3 was identified, which contains a fasciclin 1-like domain and is essential for the moss Physcomitrium patens' response to short-term rapid dehydration.

Data-Driven Network Analysis for Anomaly Traffic Detection.

Cybersecurity is a critical issue in today's internet world. Classical security systems, such as firewalls based on signature detection, cannot detect today's sophisticated zero-day attacks. Machine learning (ML) based solutions are more attractive for their capabilities of detecting anomaly traffic from benign traffic, but to develop an ML-based anomaly detection system, we need meaningful or realistic network datasets to train the detection engine.

De novo full-length transcriptome analysis of two ecotypes of Phragmites australis (swamp reed and dune reed) provides new insights into the transcriptomic complexity of dune reed and its long-term adaptation to desert environments.

Background: The extremely harsh environment of the desert is changing dramatically every moment, and the rapid adaptive stress response in the short term requires enormous energy expenditure to mobilize widespread regulatory networks, which is all the more detrimental to the survival of the desert plants themselves. The dune reed, which has adapted to desert environments with complex and variable ecological factors, is an ideal type of plant for studying the molecular mechanisms by which Gramineae plants respond to combinatorial stress of the desert in their natural state. But so far, the data on the genetic resources of reeds is still scarce, therefore most of their research has focused on ecological and physiological studies.

The specific glycerolipid composition is responsible for maintaining the membrane stability of Physcomitrella patens under dehydration stress.

Land colonization is a major event in plant evolution. Little is known about the evolutionary characteristics of lipids during this process. Here, we proved that Physcomitrella patens, a bryophyte that appeared in the early evolution of terrestrial plants, has short-term desiccation resistance.

Synthesis of Mesoporous Co₃O₄/NiCo₂O₄ Nanorods and Their Electrochemical Study.

Mesoporous Co₃O₄/NiCo₂O₄ nanorods were obtained by a hydrothermal reaction with the assistance of Ni foam and subsequent annealing treatment. The characterization of this composition by X-ray diffraction, X-ray photoelectron spectroscopy, high-resolution transmission electron microscopy, energy dispersive spectra and Brunauer-Emmett-Teller analysis revealed that the nanorods consisted of Co₃O₄ and NiCo₂O₄ phase, exhibiting high porosity and rich crystal defects. The electrochemical data showed a specific capacitance of 1173 mF cm and 606 mF cm at 2 mV s and 1 mA cm, respectively.

Cytosolic APX2 is a pleiotropic protein involved in HO homeostasis, chloroplast protection, plant architecture and fertility maintenance.

Rice cytoplasmic APX2 is a pleiotropic protein, densely distributed around chloroplasts. It plays key roles in HO homeostasis and chloroplast protection, and is related to plant architecture and fertility regulation. Ascorbate peroxidases (APXs) catalyze the conversion of HO into HO.

A Proteome Translocation Response to Complex Desert Stress Environments in Perennial Sympatric Ecotypes with Contrasting Water Availability.

After a long-term adaptation to desert environment, the perennial aquatic plant has evolved a desert-dune ecotype. The desert-dune ecotype (DR) of showed significant differences in water activity and protein distribution compared to its sympatric swamp ecotype (SR). Many proteins that were located in the soluble fraction of SR translocated to the insoluble fraction of DR, suggesting that membrane-associated proteins were greatly reinforced in DR.

Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation.

The surface plasmon resonance (SPR) effect endows gold nanoparticles (GNPs) with the ability to visualize biomolecules. In the present study, we designed and constructed a GNP probe to allow the semi-quantitative analysis of methylated tumor suppressor genes in cultured cells. To construct the probe, the GNP surfaces were coated with single-stranded DNA (ssDNA) by forming Au-S bonds.

Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability.

Green vegetative tissues of the moss Physcomitrella patens possess a powerful ability to tolerate severe drought stress. Proteomics analysis have revealed that a large number of late embryogenesis abundant (LEA) proteins were key players in the drought tolerance of the photosynthetic tissues. PpLEA4-20, a member of the moss LEA protein family, was selected for further function study using an ectopic expression method in rice.

Reduced ABA Accumulation in the Root System is Caused by ABA Exudation in Upland Rice (Oryza sativa L. var. Gaoshan1) and this Enhanced Drought Adaptation.

Lowland rice (Nipponbare) and upland rice (Gaoshan 1) that are comparable under normal and moderate drought conditions showed dramatic differences in severe drought conditions, both naturally occurring long-term drought and simulated rapid water deficits. We focused on their root response and found that enhanced tolerance of upland rice to severe drought conditions was mainly due to the lower level of ABA in its roots than in those of the lowland rice. We first excluded the effect of ABA biosynthesis and catabolism on root-accumulated ABA levels in both types of rice by monitoring the expression of four OsNCED genes and two OsABA8ox genes.

Proteomic study of the impact of Hik33 mutation in Synechocystis sp. PCC 6803 under normal and salt stress conditions.

Cyanobacteria are the only prokaryotes possessing plasma, thylakoid, and outer membranes. The plasma membrane of a cyanobacterial cell is essential for the biogenesis of cyanobacterial photosystems and serves as a barrier against environmental stress. We previously identified dozens of salt-responsive proteins in the plasma membrane of Synechocystis sp.

Proteome analysis of Physcomitrella patens exposed to progressive dehydration and rehydration.

Physcomitrella patens is an extremely dehydration-tolerant moss. However, the molecular basis of its responses to loss of cellular water remains unclear. A comprehensive proteomic analysis of dehydration- and rehydration-responsive proteins has been conducted using quantitative two-dimensional difference in-gel electrophoresis (2D-DIGE), and traditional 2-D gel electrophoresis (2-DE) combined with MALDI TOF/TOF MS.

Cd-induced changes in leaf proteome of the hyperaccumulator plant Phytolacca americana.

Cadmium (Cd) is highly toxic to all organisms. Soil contamination by Cd has become an increasing problem worldwide due to the intensive use of Cd-containing phosphate fertilizers and industrial zinc mining. Phytolacca americana L.

Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells.

The green alga Chlamydomonas reinhardtii is a model organism to study H(2) metabolism in photosynthetic eukaryotes. To understand the molecular mechanism of H(2) metabolism, we used 2-DE coupled with MALDI-TOF and MALDI-TOF/TOF-MS to investigate proteomic changes of Chlamydomonas cells that undergo sulfur-depleted H(2) photoproduction process. In this report, we obtained 2-D PAGE soluble protein profiles of Chlamydomonas at three time points representing different phases leading to H(2) production.

Proteomic characterization of Phragmites communis in ecotypes of swamp and desert dune.

Phragmites communis Trin. (common reed) is a recognized model plant for studying its adaptation to contrasting and harsh environments. To understand the inherent molecular basis for its remarkable resistance to combined stresses, we performed a comprehensive proteomic analysis of the leaf proteins from two ecotypes, i.

Proteomic analysis of plasma membranes of cyanobacterium Synechocystis sp. Strain PCC 6803 in response to high pH stress.

Cyanobacteria are unique prokaryotes possessing plasma-, outer- and thylakoid membranes. The plasma membrane of a cyanobacterial cell serves as a crucial barrier against its environment and is essential for biogenesis of cyanobacterial photosystems. Previously, we have identified 79 different proteins in the plasma membrane of Synechocystis sp.

Motion compensation via redundant-wavelet multihypothesis.

Multihypothesis motion compensation has been widely used in video coding with previous attention focused on techniques employing predictions that are diverse spatially or temporally. In this paper, the multihypothesis concept is extended into the transform domain by using a redundant wavelet transform to produce multiple predictions that are diverse in transform phase. The corresponding multiple-phase inverse transform implicitly combines the phase-diverse predictions into a single spatial-domain prediction for motion compensation.

A proteomic analysis of cold stress responses in rice seedlings.

Using proteomic analysis, an investigation aimed at a better understanding of the molecular adaptation mechanisms of cold stress was carried out in rice (Oryza sativa). The seedlings were exposed to a progressively low temperature stress treatment from normal temperature to 15, 10, and 5 degrees C. Proteins were extracted from the leaves collected from both control and stressed seedlings.

Changes of element ratios of cultured cells from dune reed under adverse environmental conditions.

Dune reed, as an ecotype of reed plant (Phragmites communis Trin.), is an ideal material for studies on the adaptations of plant to environmental conditions. Scanning electron microscope, energy-dispersive X-ray analysis, and plant tissue culture techniques were used to investigate the effect of extreme temperature, salt, and polyethylene glycol-induced osmotic stress on the intracellular elements K, Na, Ca, and Cl in cultured cells from dune reed and swamp reed (as control).