Publications by authors named "Suwan Han"
Article Synopsis
- The study focused on improving the production of a steroid derivative, 9-OHBA, using a mutant strain (MFKD) from ATCC 35855, targeting the C22 metabolic pathway.
- The mutant strain MFKDΔ achieved an 81.47% accumulation of 9-OHBA, and a double mutant MFKDΔΔ further improved selectivity to 95.13%, resulting in an impressive yield of 6.81 g/L from phytosterols, outperforming previous strains.
Phytosterol conversion into C9 non-hydroxylated derivatives through gene regulation in Mycobacterium fortuitum.
Appl Microbiol Biotechnol
December 2023
Androst-4-ene-3,17-dione (AD) and 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) are important drug intermediates that can be biosynthesized from phytosterols. However, the C9 hydroxylation of steroids via 3-ketosteroid 9α-hydroxylase (KSH) limits AD and 4-HBC accumulation. Five active KshAs, the oxidation component of KSH, were identified in Mycobacterium fortuitum ATCC 35855 for the first time.
View Article and Find Full Text PDFFunctional analysis of acyl-CoA dehydrogenases and their application to C22 steroid production.
Appl Microbiol Biotechnol
June 2023
Acyl-CoA dehydrogenase (ChsE) is involved in the steroid side-chain degradation process. However, their function in vivo remains unclear. In this study, three ChsE, ChsE1-ChsE2, ChsE3, and ChsE4-ChsE5, were identified in Mycolicibacterium neoaurum, and their functions in vivo are studied and compared with those from Mycobacterium tuberculosis in vitro.
View Article and Find Full Text PDFArticle Synopsis
- 9α-hydroxyandrost-4-ene-3,17-dione (9-OHAD) is crucial for glucocorticoid drug synthesis, but its production is hampered by degradation and by-products during phytosterol biotransformation.
- The study identified five 3-Ketosteroid-Δ-dehydrogenases (KstDs) in Mycobacterium fortuitum, with KstD2 and KstD3 notably causing the degradation of 9-OHAD.
- Enhancements in production included creating a kstD-deficient strain that improved 9-OHAD yield by over 42%, achieving a final production of 12.21 g/L and a
Addressing critical ecological issues is one of the core objectives of the Chinese National New-type Urbanization Plan (NTU). However, there is not enough research to clearly demonstrate NTU's impact on environmental pollution. There is a need to provide solid empirical evidence from evaluation of the effects of NTU on environmental pollution.
View Article and Find Full Text PDFC22 steroid drug intermediates are suitable for corticosteroids synthesis, and the production of C22 steroids is unsatisfactory due to the intricate steroid metabolism. Among the C22 steroids, 21-hydroxy-20-methyl-pregna-1,4-dien-3-one (1,4-HP) could be used for Δ-steroid drug synthesis, such as prednisolone. Nevertheless, the production of 1,4-HP remains unsatisfactory.
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