Large mitochondrial genomes in tenthredinid sawflies (Hymenoptera, Tenthredinidae).

We sequenced and assembled mitochondrial genomes of three tenthredinid sawflies (, , and ) using Oxford Nanopore Technologies' MinION. The Canu assembler produced circular assemblies (23,000-40,000 bp). Still, errors were found in the highly repetitive non-coding control region because of the fragmented DNA which led to no reads spanning the complete control region, preventing its reliable assembly.

Optimised PCR assays for detecting elusive waterfowl from environmental DNA.

For many aquatic and semiaquatic mammal, amphibian and fish species, environmental DNA (eDNA) methods are employed to detect species distribution and to monitor their presence, but eDNA is much less employed for avian species. Here, we developed primers for the detection of true geese and swan species using eDNA and optimised a PCR protocol for eDNA. We selected taiga bean goose () as our focal (sub)species and sampled water from lakes, from which the presence of taiga bean goose was visually confirmed.