[Obtaining and characteristics of domestic preparation interferon alpha-2b with prolonged effect].

Pegylated interferon alpha-2b (PEG-IFN alpha-2b) is a domestic preparation of a modified recombinant interferon alpha-2b with prolonged effect. The preparation was obtained by N-terminal pegylation of IFN alpha-2b with polyethylene glycol (PEG). This paper presents the method of PEG-IFN alpha-2b synthesis and characteristics of the obtained product.

Non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L.: titration in vivo and in vitro. Brief report.

Infectivity of non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L. was determined with bioassay technique in vivo and with plaque assay method in vitro using SCLd 135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID50 for Galleria larvae injected into hemocoel for non-cloned and 0.

Purification of free polyhedrosis virus infecting Galleria mellonella L.

Free Galleria mellonella nuclear polyhedrosis virus was purified 4000--4500 times by Tween-80 treatment and repeated sucrose density gradient centrifugation, based on the ratio of infectivity to protein content as compared to the starting virus-containing suspension.