The Neuroimmune Role of Intestinal Microbiota in the Pathogenesis of Cardiovascular Disease.

Currently, a bidirectional relationship between the gut microbiota and the nervous system, which is considered as microbiota-gut-brain axis, is being actively studied. This axis is believed to be a key mechanism in the formation of somatovisceral functions in the human body. The gut microbiota determines the level of activation of the hypothalamic-pituitary system.

Programmed death-ligand 1 signaling pathway involves in bladder cancer growth and progression.

Context: Exploration of the biological property of programmed death-ligand 1 (PD-L1) signaling that may impact bladder tumor growth in humanized animals and cell culture.

Aims: The aim of this study is to evaluate how PD-L1 signaling involves bladder cancer growth and progression.

Settings And Design: This study design involves experimental and study.

Patient-Derived Non-Muscular Invasive Bladder Cancer Xenografts of Main Molecular Subtypes of the Tumor for Anti-Pd-l1 Treatment Assessment.

Background: Establishment of heterotopic patient-derived xenografts of primary and relapsed non-muscular invasive bladder cancer (NMIBC) to explore the biological property of PD-L1 signaling that may impact bladder tumor growth in humanized animals.

Methods: Tumor cells of luminal, basal, and p53 subtypes of primary and relapsed NMIBC were engrafted to irradiated (3.5 Gy) NOG/SCID female mice along with intraperitoneal transplantation of human lymphocytes (5 × 10 cells/mouse); a role of PD-L1 signaling pathway inhibition for bladder cancer growth was assessed in humanized animals that carried PD-L1-expressing main molecular subtypes of bladder carcinoma patient-derived xenografts (PDX) and provided with selective anti-PD-L1 treatment.

Novel aminochromone derivative inhibits tumor growth on xenograft model of lung cancer in mice.

2-Amino-4H-chromene derivatives possess anticancer property proved on different and models of malignancies such breast, nasopharyngeal, bladder, ovary carcinomas, astrocytoma, and osteosarcoma. We assumed it might be effective to apply one of the derivatives as promising approach to lung carcinoma treatment. to evaluate how novel 4-aryl substituted 2-amino-4H-chromene derivative AX-554 impacts tumor growth and progression, as well as possible mechanisms for anticancer effect development on patient-derived heterotopic xenograft model of lung carcinoma in mice.

Pemphigus vulgaris treated with allogeneic hematopoietic stem cell transplantation following non-myeloablative conditioning.

Objectives: Successful treatment of severe, refractory Pemphigus vulgaris (PV) is reported.

Methods: Reduced intensity, non-myeloablative conditioning was employed, followed by allogeneic hematopoietic stem cell transplantation (HSCT) from a fully matched sibling.

Results: Treatment of refractory PV with myeloablation and subsequent allogenic HSCT has been previously reported, and sustained remission with this approach has been achieved.

[Bizerine: new anticholinesterase drug with selective gastrointestinal action].

Bizerine-oxalate of hexamethylene-bic-[N-methylcarbamic acid-3-(2-dimethylaminomethyl)pyridyl ether] exhibits the properties of acetylcholine esterase (AChE) inhibitor, being comparable in this respect in in vitro tests to aminostigmine. Bizerine is 2.5 and 6.

[The investigation of the composition of liquid radioactive waste].

In investigation the process of composition sediment of liquid unorganic radioactive waste, that are forming in cistern-selectors at PNPI RAS, it was discovered apart from great quantity of ions of different metals and radionuclides considerable maintenance of organic material (to 30% and more from volume of sediment) unknown origin. A supposition was made about its microbiological origin. Investigation shows, that the main microorganisms, setting this sediment, are the bacterious of Pseudomonas kind, capable of effectively bind in process of grow the radionuclide 90Sr, that confirms the potential posibility of using this microorganisms for bioremediation of liquid low radioactive wastes (LRW).

[Isolation and investigation of natural yeast strains resistant to heavy metal salts and radionuclides].

The collection of yeasts (more than 2000 strains) from extreme natural environment of Kamchatka peninsular and Kuril Islands was created. 448 strains were selected for their resistance to salts of such heavy metals as Cu, Cd, Co and to high temperature (37-52 degrees C). 72% of strains appeared to be resistant to one or more selective factors.

[The bactericidal activity of lectins from nitrogen-fixing bacilli].

Lectins I and II isolated from the nitrogen-fixing soil bacterium Paenibacillus polymyxa 1460 were found to be able to suppress the growth of Rhizobium leguminosarum 252 and Bacillus subtilis 36 at nearly all the concentrations tested (from 1 to 10 micrograms/ml). Lectin I was also inhibitory to Azospirillum brasilense 245 and Erwinia carotovora subsp. citrulis 603, while lectin II exerted bactericidal activity against Xanthomonas campestris B-610 and B-611 and A.

[Protection of mice by carbamates cholinesterase inhibitors against poisoning by armin and its dependence on certain physico-chemical indicators].

A series of aminostigmin derivatives with various substituents at nitrogen in the second position of the pyridine ring, has been tested. The efficacy of preventing the death of mice poisoned by armine in five of the seven substances correlates with the constant of the rate of carbamylation of acetylcholinesterase in the in vitro experiments and with the hydrophobic nature. It is suggested that the phenomenon of protection of animals against the toxic effect of organophosphorous compounds involves the "leaving portion" of the molecule of carbamates.

[Effect of lysophasphatidylcholine on sensitivity of the heart to acetylcholine and binding of quinuclidinyl benzilate to myocardial membranes].

Chemical nature of the blood serum factor exerting cholinolytic effect on the animal heart, was determined. The effect is due to the serum lipid component: lysophosphatidylcholine. The latter in concentration 1-25 microM suppresses the sensitivity of the frog perfused heart ventricle to acetylcholine.

[Quantitative analysis of inducible systems of Escherichia coli and Bacillus subtilis using radioimmunological methods].

By RIA dot-blot method two main cell system, system SOS-repair and heat shock system, was showed possibility to receive as well qualitative as quantitative results influence of different induced agents. Was showed quantitative differences in initial levels enzymes of RecA and CroEL in different strains of E. coli, as well of principle possibility to using received antibody, which has high specific to this enzymes, for investigation reactions this systems in the other bacterial species, such as Bac.

[Proteins induction by cysteamine in Escherichia coli cells].

It was shown, what in presence of oxygen in broad interval time of treatment in E. coli cells was induced line proteins by cysteamine which are proteins SOS--repair system and heat--shock system. There are quantitative defined induction level RecA, GroEL and DnaK proteins which are members this systems.

Effect of lysophosphatidylcholine on the structure and function of low density lipoproteins.

The treatment of LDL with bee-venom phospholipase A2 resulted in the formation of lipid-protein particles (phl-LDL) with an increased content of lysophosphatidylcholine (LPC). At the same time, the composition of other lipids and the protein structure remained unaffected. phl-LDL, as well as LPC, abolished the hormone-induced [Ca2+] increase in platelets and platelet aggregation induced by PAF, AMP and thrombin, whereas LDL produced no effect on the hormone-stimulated increase in the intracellular [Ca2+].

[Biochemical characteristics of aminostigmine--a new anticholinesterase agent].

The properties of aminostigmine in comparison with those of other carbamate inhibitors of cholinesterases have been studied in vitro using potentiometric titration and Ellman methods. The bimolecular constants of the inhibition rate of acetyl-, butyryl- and propionylcholinesterase were found to be equal to (8.0-14.

[Subtypes of the functionally active muscarinic receptors in the heart of the frog Rana temporaria].

Radioligand analysis of ventricular membranes from the frog Rana temporaria with muscarinic antagonist 3H-QNB (qunuclidinul benzilate) revealed homogeneous population of highly specific muscarinic receptors with high affinity (Kd = 0.502 +/- 0.020 nM and Bmax = 789 +/- 18 fmol/mg protein).

[Induction of the SOS-like system in Rec-mutants of Bacillus subtilis].

Influence of the recE1, recB2, recB3, recB19, recF15, recF18, recL16, recM13 and recM27 mutations of the induction of the SOS-like system component, i. e. the RecE protein of Bacillus subtilis was studied by RIA-dot-blot method in UV-irradiated or treated by nalidixic acid cells.

[The pharmacological characteristics of the new anticholinesterase drug aminostigmine].

The pharmacological properties of a new drug approved in the USSR for the treatment of cholinolytics poisonings are described. The drug is as active as physostigmine. However, its action is longer.

[Effect of macroergic nucleotides and lysolecithin on the cholinergic reception of the heart muscle in the frog Rana temporaria].

In experiments on isolated frog ventricle, it has been demonstrated that the dose-response curve for negative inotropic reaction of the myocardium to acetylcholine exhibits a sigmoid form, Hill's coefficient (nH) of this reaction being more than 1. The value of nH depends on the interval from isolation of the ventricle and on the duration of perfusion of the latter with Ringer's solution. It was shown that ATP, UTP, UDP and GTP in physiological concentrations induce both the increase in nH and the increase of K50 (acetylcholine concentration evoking the effect which is equal to half of the maximal one) of the investigated physiological reaction.

[Mutagenic effects of gamma-rays on plasmid DNA in Escherichia coli].

A purified and dried DNA of plasmid pKO482 (galK+) is 10 times more resistant to the inactivating action of 60Co-gamma-rays than that of lambda phage. gamma-Irradiation of the plasmid DNA induces forward mutations of galK, the frequency of which increases linearly with the dose. The efficiency of the mutagenic action of gamma-rays on the plasmid galK locus is 10(-12) per 1 rad and per 1 base pair.