Robust Protocol for the Synthesis of BSA Nanohydrogels by Inverse Nanoemulsion for Drug Delivery.

In a highly efficient and reproducible process, bovine serum albumin (BSA) nanogels are prepared from inverse nanoemulsions. The concept of independent nanoreactors of the individual droplets in the nanoemulsions allows high protein concentrations of up to 0.6% in the inverse total system.

Investigation of the Mechanism of SiO Particle and Capsule Formation at the Oil-Water Interface of Dye-Stabilized Emulsions.

In a previous contribution we described the formation of silica nanostructures in dye-stabilized nanoemulsions from tetraethyl orthosilicate droplets in water. Depending on the type of dye, either capsules (crystal violet, CV) or nanoparticles (congo red, CR) are formed. The thorough study of the sol-gel process uses a combination of time- and/or temperature-resolved small-angle X-ray scattering, transmission electron microscopy, and H NMR spectroscopy to elucidate the detailed kinetics and mechanism of structure formation.

Albumin Microspheres as "Trans-Ferry-Beads" for Easy Cell Passaging in Cell Culture Technology.

Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles.

BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing in Serum and Blood.

Systemic blood stream infections are a major threat to human health and are dramatically increasing worldwide. is a WHO-alerted multi-resistant pathogen of extreme importance as a cause of sepsis. Septicemia patients have significantly increased survival chances if sepsis is diagnosed in the early stages.

Double in Situ Preparation of Raspberry-like Polymer Particles.

Well-defined raspberry-like poly(styrene- co-4-vinylpyridine)-SiO nanocomposite particles with a diameter of around 200 nm were easily prepared by a double in situ process in nanoemulsion with the water-soluble dye Eosin Y as the stabilizer. During radical polymerization of the nanodroplets comprising styrene, 4-vinylpyridine (4-VP), and tetraethoxysilane (TEOS), the silane phase is expelled from the polymer phase to the oil/water (o/w) interface. In the later polymerization stage, SiO nanoparticles with a size of around 25 nm were produced via the in situ sol-gel reaction of TEOS at the o/w interface promoted by the negatively charged dye.

Green Chemistry in Red Emulsion: Interface of Dye Stabilized Emulsions as a Powerful Platform for the Formation of sub-20-nm SiO Nanoparticles.

Dye stabilized nanoemulsions offer the unique possibility of creating both silica capsules and sub-20-nm particles with precise control of particle size and narrow dispersity from the same system by the choice of the proper dye. The large o/w interface enhances the kinetics of particle formation significantly over macroscopic interfaces which enables the synthesis of silica nanoparticles without any catalyst or elevated temperatures under static conditions. This is in contrast to syntheses for sub-20-nm silica nanoparticles described until now which can normally not be conducted at neutral pH and/or room temperature without stirring.

Directing intracellular supramolecular assembly with N-heteroaromatic quaterthiophene analogues.

Self-assembly in situ, where synthetic molecules are programmed to organize in a specific and complex environment i.e., within living cells, can be a unique strategy to influence cellular functions.

Highly Transparent w/o Pickering Emulsions without Adjusting the Refractive Index of the Stabilizing Particles.

Pickering emulsions with a remarkable transmittance of up to 86% across the visible spectrum have been prepared without adjusting the refractive index (RI) of the stabilizing particles to those of the aqueous and oil phases. Commercially available hydrophilic silica particles with a diameter of 20 nm, which are hydrophobized partially in situ, were used to stabilize water droplets with diameters below 400 nm in IsoparM. In this system, the stabilizing particles and the emulsion droplets act as one single scattering object, which renders RI-matching of the particles unnecessary.

Alizarin Yellow R (AYR) as compatible stabilizer for miniemulsion polymerization.

Hypothesis: Many solid particles have been used in Pickering stabilized (mini)emulsions. Stabilizing "particles" can be also formed in situ e.g.

Dye Aggregates as New Stabilizers for (Mini)emulsions.

Water-soluble organic dyes such as fluorescein are widely used, mainly for coloration of, e.g., biological samples and groundwater tracing, and they are not obviously amphiphilic by molecular structure like surfactants.

Inverse Pickering Emulsions with Droplet Sizes below 500 nm.

Inverse Pickering emulsions with droplet diameters between 180 and 450 nm, a narrow droplet size distribution, and an outstanding stability were prepared using a miniemulsion technique. Commercially available hydrophilic silica nanoparticles were used to stabilize the emulsions. They were hydrophobized in situ by the adsorption of various neutral polymeric surfactants.