Quantification of florfenicol and its metabolites in fillets of Nile tilapia: Synthesis of metabolites and validation of an on-line solid-phase extraction-ultra high-performance liquid chromatography-tandem mass spectrometry method.

This study concerns the synthesis of the florfenicol (FF) metabolites florfenicol amine (FFA), florfenicol alcohol (FFOH), and monochloroflorfenicol (FFCl), for their subsequent use as reference standards in On-line solid-phase extraction-ultra high-performance liquid chromatography-tandem mass spectrometry (SPE-UHPLC-MS/MS) analysis. The metabolites were characterized using H and C NMR, as well as HRMS, and their purities were confirmed by quantitative NMR to ensure analytical reliability. Validation of the developed analytical method showed that it presented acceptable performance, with linearity >0.

Porous sulfur polymers for effective aqueous-phase organic contaminant removal.

Sulfur polymers produced through 'inverse vulcanization' exhibit various attributes, such as photocatalytic activity and a high capacity to adsorb heavy metals. Nevertheless, there is a lack of research investigating the use of sulfur polymers as materials for the removal of organic contaminants. In this work, porous sulfur polymers (PSPs) were synthesized from elemental sulfur and 1,3-diisopropenylbenzene, with porosity introduced via salt templating.

Evaluating the persistence of malachite green residues in tilapia and pacu fish.

Although banned in food-producing animals, residues of malachite green (MG) and its primary metabolite, leucomalachite green (LMG), have been found in fish due to illegal use in aquaculture and the release of industrial wastewater, which represent a serious risk to food and environmental securities. This study aimed to investigate the residue depletion profile of MG and LMG in edible tissues of Nile tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) cultured simultaneously under the same environmental conditions to support control measures in case of abuse. An analytical method involving QuEChERS sample preparation and liquid chromatography coupled to tandem mass spectrometry was developed, validated, and applied to quantify MG and LMG residues in fish fillets from two depletion experiments after treatment by immersion bath (MG at 0.

Levamisole incorporation in fish feed - Ensuring the medication dose and avoidance of leaching into the water.

Levamisole, an anthelmintic and immunostimulant drug, has been studied as a promising alternative for aquaculture use. While oral administration through feeding is the main route of administration in fish farming, no studies evaluating methods of levamisole incorporation into the feed have been reported so far. Therefore, this study aimed to evaluate potential procedures for levamisole incorporation in extruded fish feed using ethyl cellulose, gelatin, or vegetable oil, to avoid drug leaching to the water during the animal's medication.

Assessment of tropane alkaloid levels in Brazilian buckwheat flour products: A novel LC-UHPLC-MS/MS approach using solid-liquid extraction at low temperature.

In recent years, the monitoring of tropane alkaloids, specifically hyoscyamine and scopolamine, in food has become a pressing concern. This is due to increasing reports of food contamination with these compounds worldwide, raising awareness about the potential risks associated with their consumption. A novel method is proposed here for the determination of the sum of (+)-hyoscyamine, (-)-hyoscyamine, and (-)-scopolamine in buckwheat-based matrices, using solid-liquid extraction at low temperature and quantification by bidimensional chromatography coupled to tandem mass spectrometry.

Synthesis and characterization of TiO-carbon filter materials for water decontamination by adsorption-degradation processes.

Wastewater treatment is becoming ever more challenging due to the increasing levels of molecular pollutants that are challenging for existing approaches. Innovative materials are required to help produce potable water from heavily contaminated water sources. One such material is titanium dioxide-activated carbon (TiO/AC) heterostructures, which combine the photocatalytic properties of TiO with the adsorption properties of the ACs.

A comprehensive LC-UHPLC-MS/MS method for the monitoring of N-nitrosamines in lipophilic drugs: A case study with rifampicin.

In the last five years, the presence of N-nitrosamines in commonly used medicines has become a significant concern for patients, physicians, and the pharmaceutical industry, due to their carcinogenic properties, even at low concentrations. Analytical methods that enable the unequivocal monitoring of these compounds, with low detection limits and covering a range of drugs, are indispensable. The present work proposes a bidimensional liquid chromatography-tandem mass spectrometry method capable of quantifying eleven N-nitrosamines in lipophilic active pharmaceutical ingredients (APIs).

Adsorption of aqueous phase contaminants of emerging concern by activated carbon: Comparative fixed-bed column study and in situ regeneration methods.

This work investigated the adsorption of five model contaminants of emerging concern (CECs) that are released daily in domestic effluents (caffeine, hydrochlorothiazide, saccharin, sulfamethoxazole and sucralose) onto two activated carbons (ACs), in fixed-bed column experiments with different aqueous matrices (ultrapure water, wastewater treatment plant (WWTP) effluent and WWTP effluent pretreated by reverse osmosis and photoperoxidation (reuse water)). The ACs were chemically similar, but AC1 had smaller particles (0.7-1.

Adsorption of recalcitrant contaminants of emerging concern onto activated carbon: A laboratory and pilot-scale study.

According to the World Health Organization (WHO), the definition of water quality indicators, including contaminants of emerging concern (CECs), associated with the development of multi-barrier approaches for wastewater treatment, are crucial steps towards direct potable reuse of water. The aims of this study were 1) quantifying twelve CECs (including pharmaceutical, stimulant, and artificial sweetener compounds) in both untreated and treated wastewater samples in a Brazilian wastewater treatment plant (WWTP) using bidimensional liquid chromatography coupled with tandem mass spectrometry, allowing the selection of five marker (i.e.

Competitive kinetics of adsorption onto activated carbon for emerging contaminants with contrasting physicochemical properties.

Activated carbon (AC) can be used for the removal of emerging contaminants (e.g., drugs) in water and wastewater treatment plants.

On-line solid phase extraction-ultra-high performance liquid chromatography coupled to tandem mass spectrometry for the determination of N-nitrosodiethanolamine in baby shampoo.

N-nitrosodiethanolamine (NDELA) is a carcinogenic contaminant of concern in the cosmetics industry. Contaminated raw material, degradation, reactions of ingredients of the formulation, or migration of packaging material can be responsible for the presence of NDELA in the final product. Liquid chromatography coupled to tandem mass spectrometry is the most widely accepted technique for the quantitation of NDELA in cosmetic products.

Removal of low-calorie sweeteners at five Brazilian wastewater treatment plants and their occurrence in surface water.

The consumption of low-calorie sweeteners (LCSs) such as acesulfame (ACE), sucralose (SUC), saccharin (SAC), cyclamate (CYC), aspartame (ASP), neotame (NEO), and stevioside (STV) is increasing worldwide to meet the demand for reduced-calorie foods and beverages. However, there are no consumption data available in Brazil, as well as their concentration in sewage and removal on wastewater treatment plants (WWTPs). In the present study, ACE, SUC, SAC, CYC, ASP, NEO, and STV were assessed at five WWTPs located in the metropolitan region of Campinas (São Paulo State, Brazil), in operation with different treatment processes.

Long-term ecotoxicological effects of ciprofloxacin in combination with caffeine on the microalga .

Ciprofloxacin (CIP) is an antimicrobial "pseudo-persistent" in aquatic ecosystems. Once dispersed in the water compartments, it can also affect the microalgae. Thus, the evaluation of its long-term ecotoxicological effects is necessary.

Degradation of antidepressant pharmaceuticals by photoperoxidation in diverse water matrices: a highlight in the evaluation of acute and chronic toxicity.

Photoperoxidation (UV/HO) was used to degrade three of the worldwide most consumed antidepressant pharmaceuticals-bupropion, escitalopram, and fluoxetine-in ultrapure water, drinking tap water, surface water, and reclaimed water. The study was performed with antidepressants in concentration levels in which these compounds usually occur in the water matrices. Online solid-phase extraction coupled to UHPLC-MS/MS was used to quantify the analytes during degradation studies.

Tracking the occurrence of psychotropic pharmaceuticals in Brazilian wastewater treatment plants and surface water, with assessment of environmental risks.

According to the World Health Organization, >360 million people worldwide suffer from mental diseases such as depression, anxiety, or bipolar disorder, for which psychotropic drugs are frequently prescribed. Despite being highly metabolized in the human organism, non-metabolized portions of these drugs are excreted, subsequently reaching wastewater treatment plants (WWTPs), where they may be incompletely removed during treatment, leading to the contamination of surface waters. In this work, ten psychotropic drugs widely consumed in Brazil (alprazolam, amitriptyline, bupropion, carbamazepine, clonazepam, escitalopram, fluoxetine, nortriptyline, sertraline, and trazadone) were monitored at five WWTPs located in the metropolitan region of Campinas (São Paulo State, Brazil).

Leaching of benzimidazole antiparasitics in soil columns and in soil columns amended with sheep excreta.

Benzimidazoles are anthelmintics frequently used in sheep farming due to the high susceptibility of these animals to parasitic diseases. Sheep excreta are often disposed onto soils as a fertilizer, and they may contain benzimidazole residues that can contaminate soil and water. This work aimed to assess the leaching behavior of benzimidazole drugs (albendazole, fenbendazole, and thiabendazole) and their metabolites in two Brazilian soils of different textural classifications (sandy and clay), as well as sheep excreta-amended soils, following the OECD 312 Guidelines.

On-Flow LC-MS/MS method for screening of xanthine oxidase inhibitors.

The screening of compounds is the initial step in research for the development of new drugs. For this reason, the availability of fast and reliable tools for the screening of a large number of compounds becomes essential. Among the therapeutic targets, the enzyme xanthine oxidase (XO) is of great interest for its importance as a biological source of superoxide radicals, which contribute to the oxidative stress on organisms and are involved in many pathological processes.

Aerobic dissipation of avermectins and moxidectin in subtropical soils and dissipation of abamectin in a field study.

Avermectins and moxidectin are antiparasitics widely used as active pharmaceutical ingredients in veterinary medicine, as well as in pesticide formulations for pest control in agriculture. Although the use of these compounds provides benefits to agribusiness, they can impact the environment, since a large part of these substances may reach the soil and water from the excreta of treated animals and following direct applications to crops. The present work had the objective of evaluating the dissipation behaviors of abamectin, doramectin, eprinomectin, ivermectin, and moxidectin in four native Brazilian soils of different textural classes (clay, sandy-clay, sandy, and sandy-clay-loam), following OECD Guideline 307.

Oral administration of antibiotics increased the potential mobility of bacterial resistance genes in the gut of the fish Piaractus mesopotamicus.

Background: Aquaculture is on the rise worldwide, and the use of antibiotics is fostering higher production intensity. However, recent findings suggest that the use of antibiotics comes at the price of increased antibiotic resistance. Yet, the effect of the oral administration of antibiotics on the mobility of microbial resistance genes in the fish gut is not well understood.

Benzimidazoles in wastewater: Analytical method development, monitoring and degradation by photolysis and ozonation.

Pharmaceutical residues are constantly released into natural waters, mainly from wastewater treatment plants (WWTPs) whose processes are unable to completely eliminate them. Among these drugs, the occurrence of benzimidazoles, a class of antiparasitics for human and veterinary use, has been reported in WWTP effluents and surface waters. In this study, an SPE-UHPLC-MS/MS method was developed and optimized for extraction and quantitation of benzimidazoles in influents and effluents of a local WWTP and in hospital wastewater.

Sorption behaviors of antimicrobial and antiparasitic veterinary drugs on subtropical soils.

Brazil is one of the world's largest producers of animal protein, requiring the large-scale use of veterinary drugs. The administration of antimicrobials and antiparasitics is a common practice. However, there is a lack of information on how these drugs impact the environment.

Sorption mechanism of enrofloxacin on humic acids extracted from Brazilian soils.

Veterinary antimicrobials are emerging environmental contaminants of concern. In this study, the sorption of enrofloxacin (ENR) onto humic acids (HAs) extracted from three Brazilian soils was evaluated. HAs were characterized by elemental analysis and solid C nuclear magnetic resonance spectroscopy.

On-line solid-phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry for the determination of avermectins and milbemycin in soils.

Avermectins and milbemycin are widely used as veterinary drugs and as agricultural pesticides, and their residues have been detected in soil. This study reports a simple and high-throughput method for determining ivermectin (IVER), abamectin (ABA), doramectin (DORA), eprinomectin (EPRI), and moxidectin (MOXI) residues in soils, employing an on-line solid-phase extraction technique coupled with ultra-high performance liquid chromatography and tandem mass spectrometry (SPE-UHPLC-MS/MS). The method was validated and applied for the determination of ABA in soils from an orange plantation treated with this pesticide.

Bee pollen as a bioindicator of environmental pesticide contamination.

Honeybees and bee products are potential bioindicators of the presence of contaminants in the environment, enabling monitoring of large areas due to the long distances travelled by bees. This work evaluates the use of bee pollen as a bioindicator of environmental contamination by pesticides. A GC-MS/MS analytical method for multiresidue determination of 26 different pesticides in pollen was developed and validated in accordance with the recommendations of the European Union SANCO guide.