Publications by authors named "Susanne Muller-Knapp"
Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used to immobilize an antigen during phage display selections of binders. Here we describe a method of in vivo biotinylation of proteins during expression in E.
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- 2-Oxoglutarate (2OG) and iron-dependent oxygenases are important targets for treating human diseases, and this study evaluates two new inhibitors, IOX1 and 4C8HQ, against common inhibitors like NOG and 2,4-PDCA.
- The findings show that IOX1 effectively inhibits a wide range of 2OG oxygenases, including those related to histone and nucleic acid demethylation, and works in both cytosolic and nuclear environments without needing chemical modifications.
- Additionally, unique crystallographic studies reveal that IOX1 can cause significant movement of an active site metal in the oxygenases, which is an unusual phenomenon not seen with 4C8HQ.
Fumarate hydratase catalyzes the stereospecific hydration across the olefinic double bond in fumarate leading to L-malate. The enzyme is expressed in mitochondrial and cytosolic compartments, and participates in the Krebs cycle in mitochondria, as well as in regulation of cytosolic fumarate levels. Fumarate hydratase deficiency is an autosomal recessive trait presenting as metabolic disorder with severe encephalopathy, seizures and poor neurological outcome.
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