Publications by authors named "Susanna M Lissek"
Solid cancers frequently relapse with distant metastasis, despite local and systemic treatment. Cellular dormancy has been identified as an important mechanism underlying drug resistance enabling late relapse. Therefore, relapse from invisible, minimal residual cancer of seemingly disease-free patients call for in vitro models of dormant cells suited for drug discovery.
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- Monitoring tumor growth dynamics is essential for cancer research, and patient-specific tumor organoids were created from individuals with intrahepatic cholangiocarcinoma (iCCA) to assess growth continuously.
- Organoid growth was tracked over 48 hours using brightfield imaging, with results validated through a quantitative MTS assay and Ki67 immunohistochemistry to evaluate proliferation rates.
- The study found that iCCA organoid growth was significantly inhibited by the drug sorafenib in a time- and dose-dependent manner, while non-tumor organoids remained unaffected, highlighting the method's potential for personalized treatment options.
Background: Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective cell behaviour in mammalian organs during development, homeostasis, regeneration, and pathogenesis. To investigate the underlying cell organisation principles of organoids, we imaged hundreds of pancreas and cholangiocarcinoma organoids in parallel using light sheet and bright-field microscopy for up to 7 days.
Results: We quantified organoid behaviour at single-cell (microscale), individual-organoid (mesoscale), and entire-culture (macroscale) levels.