Publications by authors named "Susanna M Dang"
Acute injury in the airways or the lung activates local progenitors and stimulates changes in cell-cell interactions to restore homeostasis, but it is not appreciated how more distant niches are impacted. We utilized mouse models of airway-specific epithelial injury to examine secondary tissue-wide alveolar, immune, and mesenchymal responses. Single-cell transcriptomics and validation revealed transient, tissue-wide proliferation of alveolar type 2 (AT2) progenitor cells after club cell-specific ablation.
View Article and Find Full Text PDFAlveolar type 2 (AT2) cells, the epithelial progenitor cells of the distal lung, are known to be the prominent cell of origin for lung adenocarcinoma. The regulatory programs that control chromatin and gene expression in AT2 cells during the early stages of tumor initiation are not well understood. Here, we dissected the response of AT2 cells to Kras activation and p53 loss (KP) using combined single cell RNA and ATAC sequencing in an established tumor organoid system.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates mitotically stable random monoallelic gene expression (RME) in certain autosomal genes, focusing on the role of enhancers in this process using a mouse model and single-cell analysis of NK cell receptor genes.
- - Findings show that enhancers can be accessible and active in both silent and expressed alleles, indicating that enhancer activation and gene expression can operate independently.
- - The research suggests that many genes may exhibit RME as a result of general enhancer-driven gene regulation, challenging the idea that certain lineage-defining genes are universally expressed and positioning RME as part of a broader continuum of gene regulation behaviors.