A role for the scaffolding adapter GAB2 in breast cancer.

The scaffolding adapter GAB2 maps to a region (11q13-14) commonly amplified in human breast cancer, and is overexpressed in breast cancer cell lines and primary tumors, but its functional role in mammary carcinogenesis has remained unexplored. We found that overexpression of GAB2 (Grb2-associated binding protein 2) increases proliferation of MCF10A mammary cells in three-dimensional culture. Coexpression of GAB2 with antiapoptotic oncogenes causes lumenal filling, whereas coexpression with Neu (also known as ErbB2 and HER2) results in an invasive phenotype.

Globular domains 4/5 of the laminin alpha3 chain mediate deposition of precursor laminin 5.

In epidermal wounds, precursor laminin 5 (alpha3beta3gamma2) is deposited in the provisional basement membrane (PBM) before other BM components. Precursor laminin 5 contains G4/5 globular domains at the carboxyl terminus of the alpha3 chain. Here, the function of G4/5 was evaluated in deposition of laminin 5.

Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies.

Historically, Mabs have been one of the most productive and reliable methods for the identification of adhesion receptors and adhesive ECM ligands. In large part, this is because Mabs can identify the function of the adhesion components within the context of the complex ECM or the cell surface. There are now many isoforms of laminin, collagen, and other ECM components that have been identified by molecular and Mab approaches.