Clinical Validation of the Vitro HPV Screening Assay for Its Use in Primary Cervical Cancer Screening.

Many scientific societies have issued guidelines to introduce population-based cervical cancer screening with HPV testing. The Vitro HPV Screening assay is a fully automatic multiplex real-time PCR test targeting the L1 GP5+/GP6+ region of HPV genome. The assay detects 14 high risk (HR) HPV genotypes, identifying individual HPV16 and HPV18 genotypes, and the HPV-positive samples for the other 12 HR HPV types are subsequently genotyped with the HPV Direct Flow Chip test.

Effectiveness of self-applied acupressure for cervical pain of benign origin (EDIDO-CUH): a randomized controlled clinical trial.

Background: Cervical pain is a problem with a high prevalence of ~13% of the population, and is more common in women (16.5%). The most affected age group is 65-74 years.

Adiponectin gene variant RS rs266729: Relation to lipid profile changes and circulating adiponectin after bariatric surgery.

Background: ADIPOQ rs266729 have been associated with body mass index and metabolic parameters.

Objectives: Our aim was to assess the contribution of this genetic variant on lipid profile and serum adiponectin levels after biliopancreatic diversion surgery in morbidly obese patients in a 3-year prospective study.

Setting: Tertiary Hospital.

Contributions of trace elements to the sea by small uncontaminated rivers: Effects of a water reservoir and a wastewater treatment plant.

Trace element contributions from small rivers to estuaries is an issue barely addressed in the literature. In this work, freshwater flowing into the Ria of Cedeira (NW Iberian Peninsula) was studied during a hydrological year through the input from three rivers, one considered uncontaminated (the Das-Mestas River), a second affected by urban treated wastewater discharges (the Condomiñas River), and the third containing a water reservoir for urban supply (the Forcadas River). With the objective of assessing the possible influence of human pressure, the annual yields for selected trace elements (Al, Fe, As, Cd, Co, Cr, Cu, Mn, Mo, Ni and Pb) were estimated and compared by normalizing by basin surface.

Apoptotic efficacy of etomoxir in human acute myeloid leukemia cells. Cooperation with arsenic trioxide and glycolytic inhibitors, and regulation by oxidative stress and protein kinase activities.

Fatty acid synthesis and oxidation are frequently exacerbated in leukemia cells, and may therefore represent a target for therapeutic intervention. In this work we analyzed the apoptotic and chemo-sensitizing action of the fatty acid oxidation inhibitor etomoxir in human acute myeloid leukemia cells. Etomoxir caused negligible lethality at concentrations up to 100 µM, but efficaciously cooperated to cause apoptosis with the anti-leukemic agent arsenic trioxide (ATO, Trisenox), and with lower efficacy with other anti-tumour drugs (etoposide, cisplatin), in HL60 cells.